The Biophysics Collaborative Access Team (SAXS Core)

生物物理学协作访问团队（SAXS 核心）

• 批准号: 10032575
• 负责人: Srinivas Chakravarthy
• 金额: $ 59.4万
• 依托单位: ILLINOIS INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-02-01 至 2025-12-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10032575
• 关键词: Adopted; Automation; Behavior; Binding; Biochemical; Biocompatible Materials; Biological; Biophysics; Blood capillaries; Categories; Cell physiology; Cells; Collaborations; Communities; Computer software; Data; Data Analyses; Data Collection; Data Storage and Retrieval; Development; Enzymes; Equilibrium; Felis catus; Funding; Future; Heterogeneity; Human Resources; International; Ionic Strengths; Ligand Binding; Liquid Chromatography; Liquid substance; Logistics; Maintenance; Microfluidics; Midwestern United States; Molecular Sieve Chromatography; Network Infrastructure; Nucleic Acid Folding; Performance; Proteins; Publications; Radiation induced damage; Recording of previous events; Resource Development; Resources; Roentgen Rays; Sampling; Site; Software Tools; Structural Biologist; Structure; Synchrotrons; System; Technology; Time; United States National Institutes of Health; base; beamline; biophysical tools; data acquisition; data analysis pipeline; design; detector; enzyme substrate; experimental study; improved; instrument; instrumentation; interest; light scattering; macromolecule; millisecond; multimodality; open data; operation; programs; time resolved data; user friendly software

The primary objective of the SAXS program at BioCAT is to provide the biomedical community access to the small angle x-ray scattering instrument and to maintain the state-of-the-art. BioCAT has nurtured a user program with a rich history of productive collaborations that include a diverse set of biomedical interests. User support is provided 24/7 for onsite users, and BioCAT provides remote collaborations that involve a high degree of beamline personnel participation. There are two broad categories of experiments performed under the SAXS program: equilibrium and time-resolved SAXS. Liquid chromatography SAXS (LC-SAXS) constitutes > 90% of the equilibrium SAXS program. LC-SAXS in combination with a coflow sample cell, based on a design first adopted by the Australian SAXS beamline, has streamlined SAXS data acquisition at BioCAT by nullifying common concerns such as sample heterogeneity and radiation damage induced capillary fouling. Introduction of in-line multi-angle light scattering (MALS) and dynamic light scattering (DLS) detectors has further enhanced the scope and quality of data acquired using our equilibrium SAXS instrument. As part of the time-resolved SAXS program, three different options are available which cater to time regimes from ~80 microseconds to several seconds. The stopped-flow mixer or a laminar-flow mixer are recommended for accessing time regimes between a millisecond and a few seconds, depending on the amount of sample available and the system being studied. The chaotic flow mixer is used to access earlier time regimes (~ 80µs to ~ 90 ms). Optimized fluid delivery systems, coordinated triggering mechanisms, and GUI based user-friendly software tools developed in house have now made the continuous flow time-resolved SAXS instrument at BioCAT one of its kind in the world and open to the general user community. BioCAT also maintains the widely used open-source data analysis program BioXTAS RAW. Maintenance and upgrade plans cover improved liquid chromatography systems, data analysis software, and microfluidic mixers, as well as upgraded computing, data storage capabilities, network infrastructure, and detectors. We will fully optimize the instrument to take advantage of the world class beam that will be provided by the APS-U project. Last but not the least we will continue to increase automation for SAXS data acquisition in order to make the user as independent as possible.

BioCAT 的 SAXS 计划的主要目标是为生物医学界提供获取 小角度X射线散射仪并保持最先进的水平BioCAT培养了用户。 该计划具有丰富的富有成效的合作历史，其中包括不同的生物医学兴趣。 为现场用户提供 24/7 全天候支持，BioCAT 提供远程协作，涉及高 光束线人员参与程度有两大类实验。 SAXS程序：平衡和时间分辨SAXS 液相色谱SAXS（LC-SAXS）的构成。 > 90% 的平衡 LC-SAXS 与 coflow 样品池相结合，基于 澳大利亚 SAXS 光束线首次采用的设计简化了 BioCAT 的 SAXS 数据采集 消除样品异质性和辐射损伤引起的毛细管污垢等常见问题。 在线多角度光散射 (MALS) 和动态光散射 (DLS) 检测器的推出 进一步提高了使用我们的 SAXS 仪器获取的平衡数据的范围和质量。 时间分辨 SAXS 程序，提供三种不同的选项，可满足 ~80 的时间范围 建议使用停流混合器或层流混合器。 访问时间范围在毫秒到几秒之间，具体取决于样本量 可用且正在研究的系统使用混沌流混合器来访问较早的时间状态（~ 80μs）。 至约 90 毫秒）优化的流体输送系统、协调的触发机制和基于 GUI 的用户友好型 内部开发的软件工具现已使连续流时间分辨 SAXS 仪器在 BioCAT 是世界上同类产品之一，向广大用户社区开放，BioCAT 也保持着广泛的应用。 使用开源数据分析程序 BioXTAS RAW 维护和升级计划得到了改进。 液相色谱系统、数据分析软件和微流体混合器，以及升级版 我们将全面优化计算、数据存储能力、网络基础设施和探测器。 仪器利用 APS-U 项目提供的世界级光束。 至少我们将继续提高 SAXS 数据采集的自动化程度，以使用户能够 尽可能独立。