The Biophysics Collaborative Access Team (SAXS Core)

生物物理学协作访问团队（SAXS 核心）

• 批准号: 10032575
• 负责人: Srinivas Chakravarthy
• 金额: $ 59.4万
• 依托单位: ILLINOIS INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-02-01 至 2025-12-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10032575
• 关键词: Adopted; Automation; Behavior; Binding; Biochemical; Biocompatible Materials; Biological; Biophysics; Blood capillaries; Categories; Cell physiology; Cells; Collaborations; Communities; Computer software; Data; Data Analyses; Data Collection; Data Storage and Retrieval; Development; Enzymes; Equilibrium; Felis catus; Funding; Future; Heterogeneity; Human Resources; International; Ionic Strengths; Ligand Binding; Liquid Chromatography; Liquid substance; Logistics; Maintenance; Microfluidics; Midwestern United States; Molecular Sieve Chromatography; Network Infrastructure; Nucleic Acid Folding; Performance; Proteins; Publications; Radiation induced damage; Recording of previous events; Resource Development; Resources; Roentgen Rays; Sampling; Site; Software Tools; Structural Biologist; Structure; Synchrotrons; System; Technology; Time; United States National Institutes of Health; base; beamline; biophysical tools; data acquisition; data analysis pipeline; design; detector; enzyme substrate; experimental study; improved; instrument; instrumentation; interest; light scattering; macromolecule; millisecond; multimodality; open data; operation; programs; time resolved data; user friendly software

The primary objective of the SAXS program at BioCAT is to provide the biomedical community access to the small angle x-ray scattering instrument and to maintain the state-of-the-art. BioCAT has nurtured a user program with a rich history of productive collaborations that include a diverse set of biomedical interests. User support is provided 24/7 for onsite users, and BioCAT provides remote collaborations that involve a high degree of beamline personnel participation. There are two broad categories of experiments performed under the SAXS program: equilibrium and time-resolved SAXS. Liquid chromatography SAXS (LC-SAXS) constitutes > 90% of the equilibrium SAXS program. LC-SAXS in combination with a coflow sample cell, based on a design first adopted by the Australian SAXS beamline, has streamlined SAXS data acquisition at BioCAT by nullifying common concerns such as sample heterogeneity and radiation damage induced capillary fouling. Introduction of in-line multi-angle light scattering (MALS) and dynamic light scattering (DLS) detectors has further enhanced the scope and quality of data acquired using our equilibrium SAXS instrument. As part of the time-resolved SAXS program, three different options are available which cater to time regimes from ~80 microseconds to several seconds. The stopped-flow mixer or a laminar-flow mixer are recommended for accessing time regimes between a millisecond and a few seconds, depending on the amount of sample available and the system being studied. The chaotic flow mixer is used to access earlier time regimes (~ 80µs to ~ 90 ms). Optimized fluid delivery systems, coordinated triggering mechanisms, and GUI based user-friendly software tools developed in house have now made the continuous flow time-resolved SAXS instrument at BioCAT one of its kind in the world and open to the general user community. BioCAT also maintains the widely used open-source data analysis program BioXTAS RAW. Maintenance and upgrade plans cover improved liquid chromatography systems, data analysis software, and microfluidic mixers, as well as upgraded computing, data storage capabilities, network infrastructure, and detectors. We will fully optimize the instrument to take advantage of the world class beam that will be provided by the APS-U project. Last but not the least we will continue to increase automation for SAXS data acquisition in order to make the user as independent as possible.

BioCat的SAXS计划的主要目的是为生物医学社区提供访问 小角度X射线散射仪器并维护最新的。 BioCat护理了用户 具有丰富的产品合作历史的计划，其中包括一系列生物医学兴趣。用户 现场用户24/7全天候提供支持，BioCat提供了远程协作，涉及高度 梁线人员的参与程度。在 SAXS计划：平衡和时间分辨的萨克斯。液相色谱SAXS（LC-SAXS）构成 > 90％的平衡萨克斯计划。基于A 设计首先采用的澳大利亚萨克斯横梁线已通过 消除了常见问题，例如样品异质性和辐射损伤引起的毛细血管结垢。 引入在线多角度散射（MAL）和动态光散射（DLS）检测器的引入 进一步增强了使用我们的等效萨克斯仪器获得的数据范围和质量。作为一部分 时间分辨的SAXS计划，可以使用三种不同的选项，可满足约80的时间制度 微秒至几秒钟。建议使用停止流量混合器或层流搅拌机 根据样本的数量，访问毫秒和几秒钟之间的时间制度 可用，系统正在研究。混乱的流动混合器用于访问较早的时间机制（〜80µs 优化的流体输送系统，协调的触发机制和基于GUI的用户友好 内部开发的软件工具现在使连续的流动时间分辨的萨克斯仪器处于 BioCat世界上同类产品之一，向普通用户社区开放。 BioCat也可以广泛维护 使用的开源数据分析程序Bioxtas Raw。维护和升级计划涵盖了改善 液相色谱系统，数据分析软件和微流体混合器以及升级 计算，数据存储功能，网络基础架构和检测器。我们将完全优化 利用APS-U项目将提供的世界一流梁的仪器。最后但不是 至少我们将继续增加SAXS数据采集的自动化，以使用户成为 尽可能独立。