NITROGENASE METALLOCLUSTERS & COMPLEX FORMATION

固氮酶金属簇

• 批准号: 6586738
• 负责人: DOUGLAS CHARLES REES
• 金额: $ 14.32万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-03-01 至 2003-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6586738
• 关键词: bacteria; bioimaging /biomedical imaging; biomaterials; biomedical resource; enzymes; proteins; radiography; structural biology

DNA polymerase is the enzyme responsible for the replication of the genome and repair of DNA molecule in cells. Both mesophilic and thermophilic polymerase proteins have been solved but no whole polymerase from a hyperthermophile has been completed. The structural data from a solved crystal would give more data with which to improve their understanding of the polymerase structure with respect to its activities such as DNA binding the two exonuclease activities and polymerase activity. The structure may help in expanding our basic understanding of intramolecular interaction which contribute to the thermostability of this hyperthermophilic protein.

DNA聚合酶是负责复制的酶 细胞中DNA分子的基因组和修复。 两者均可和 嗜热聚合酶蛋白已经解决，但没有整体 来自热疗法的聚合酶已经完成。 结构 来自解决晶体的数据将提供更多的数据以改进 他们对聚合酶结构的理解 诸如DNA绑定两种核酸酶活动等活动和 聚合酶活性。 该结构可能有助于扩大我们的基本 理解分子内相互作用，这有助于 这种超疗蛋白的热稳定性。