CORE--CELL SCIENCE

核心--细胞科学

• 批准号: 6440531
• 负责人: KENNETH S.K. TUNG
• 金额: $ 17.42万
• 依托单位: UNIVERSITY OF VIRGINIA
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-04-01 至 2002-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6440531
• 关键词: apoptosis; bioimaging /biomedical imaging; biomedical facility; confocal scanning microscopy; cytology; digital imaging; electron microscopy; fluorescence microscopy; histology; histopathology; image processing; immunocytochemistry; in situ hybridization; messenger RNA; method development; protein localization; single cell analysis; tissue /cell preparation; tissue resource /registry

The Cell Science Core (CSC) is an integral part of the U54 Center, providing services to its investigators and to the reproductive biology researchers at the University of Virginia by funded by the NICHD RBS Branch. The main goal of the CSC is to accomplish projects that rely on tissue and cellular localization of antigenic molecules and messenger RNA (mRNA), as well as morphologic analysis of pathologic tissue and cellular changes. Dr. Kenneth Tung, a human and experimental pathologist with expertise in reproduction and immunology, will continue to direct the CSC. He will consult or collaborate on studies requiring preparation of high quality tissue sections suitable for histology, immunohistology, immunocytology, in situ hybridization and apoptotic cell detection, as well as tissue and cellular localization of antigenic molecules and interpretation of histopathology of animal tissues. He will work with Dr. Mark Stoler, Co-director, a pathologist and an expert in situ hybridization who will consult or collaborate on studies requiring mRNA detection and quantitation. They will organize, prioritize, and quality- control the work of Ms. Melissa Bevard, an experienced histologist and Ms. Mary McCanna, an in situ hybridization technologist. When appropriate, they will implement the transfer to investigators' laboratories techniques through training of researchers, technicians, and monitoring of their work. The CSC will also develop new technology in response to and in anticipation of, research needs that will evolve in the U54. Examples include: 1) double-antibody labeling technique and double-antibody/in situ labeling technique, 2) confocal microscopy, 3) acquisition of reagents and techniques for human translational and murine reproduction research. External consultants have been recruited to serve the CSC to support this endeavor. The Core will be adapted to meet the changing research needs of the new U54 context. The P30-CSC unit has successfully provided quality service to a growing number of reproductive biology researcher, responsible for the completion of over 50 research projects and acquisition of numerous NIH grant proposals.

细胞科学核心 (CSC) 是 U54 中​​心的一个组成部分， 为其研究人员和生殖生物学提供服务 弗吉尼亚大学的研究人员，由 NICHD RBS 资助 分支。 CSC 的主要目标是完成依赖于 抗原分子和信使 RNA 的组织和细胞定位 (mRNA)，以及病理组织和细胞的形态学分析 变化。 Kenneth Tung 博士，一位人类和实验病理学家 生殖和免疫学方面的专业知识将继续指导 CSC。 他将就需要准备高水平的研究进行咨询或合作 适用于组织学、免疫组织学、 免疫细胞学、原位杂交和凋亡细胞检测等 以及抗原分子的组织和细胞定位 动物组织的组织病理学的解释。他将与博士一起工作。 Mark Stoler，联合主任、病理学家和现场专家 杂交谁将咨询或合作需要 mRNA 的研究 检测和定量。他们将组织、确定优先顺序并提高质量—— 控制经验丰富的组织学家 Melissa Bevard 女士和 玛丽·麦卡纳（Mary McCanna），原位杂交技术专家。在适当的时候， 他们将实施向研究人员实验室转移技术 通过对研究人员、技术人员的培训以及对他们的监测 工作。 CSC 还将开发新技术来应对和解决 对 U54 中​​将发展的研究需求的预期。示例 包括：1）双抗体标记技术和双抗体/原位技术 标记技术，2) 共焦显微镜，3) 试剂的获取和 人类翻译和小鼠生殖研究技术。 已聘请外部顾问为 CSC 提供服务以支持此项工作 努力。核心将进行调整以满足不断变化的研究需求 新的 U54 环境。 P30-CSC装置已成功提供质量 服务于越来越多的生殖生物学研究者， 负责完成科研项目50余项 获得许多 NIH 拨款提案。