Gene Targeting in Drosophila

果蝇基因打靶

• 批准号: 6603556
• 负责人: KENT G GOLIC
• 金额: $ 6.36万
• 依托单位: UNIVERSITY OF UTAH
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-09-01 至 2005-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6603556
• 关键词: Drosophilidae; biotechnology; gene mutation; gene targeting; genetic manipulation; genetic mapping; genetic recombination; genetic techniques; technology /technique development; transfection /expression vector

DESCRIPTION (provided by applicant): The ultimate goal of a genome project must be to understand what the genes are doing, not merely identify their existence. We are yet some distance from achieving that goal in Drosophila. The availability of a gene targeting method is but the first step towards that goal. Ultimately, it would be desirable to have a knockout mutation for every gene in the fly to be able to elucidate the function of each of those 13,600 genes. The targeting method that has been developed is vastly more efficient and effective than prior methods that have been developed for flies, but the method is in its infancy. Many parameters of the system are uncharacterized, and it is currently a lengthy procedure, requiring approximately 4-6 months from design of the targeting construct to the production of a knockout mutation. The proposed work is designed to increase our knowledge of the parameters that affect the efficiency of the method, leading to more efficient construct design and manipulation of the flies. A variety of methods to make gene knockouts will be tested and characterized to find those that are efficient and generally suitable for introducing mutations into specific genes. Experiments will also be undertaken to develop methods to speed up the process of making mutations, by weeks or perhaps months. The result of this work will provide a guide to the most efficient route towards producing specific alterations in the Drosophila genome. Moreover, the results will determine whether it becomes feasible to contemplate a large-scale knockout project in Drosophila, and what the time scale of such a project will be. Finally, the availability of efficient methods for gene targeting in Drosophila will aid the use of Drosophila as a model for understanding the biology of human diseases.

描述（由申请人提供）：基因组计划的最终目标必须 理解基因在做什么，而不仅仅是识别它们的存在。 我们距离在果蝇中实现这一目标还有一段距离。这 基因打靶方法的可用性只是实现这一目标的第一步 目标。最终，希望每个基因都具有敲除突变。 果蝇中的基因能够阐明这 13,600 个基因中每一个的功能 基因。已开发的靶向方法更加有效 比之前为果蝇开发的方法更有效，但是 方法还处于起步阶段。系统的许多参数是未表征的， 目前这是一个漫长的过程，大约需要4-6个月 从靶向结构的设计到敲除的产生 突变。拟议的工作旨在增加我们对 影响方法效率的参数，从而提高效率 构建苍蝇的设计和操纵。多种制作方法 基因敲除将被测试和表征，以找到那些 有效且通常适合将突变引入特定基因。 还将进行实验来开发加快这一过程的方法 进行突变，需要几周甚至几个月。这项工作的结果将 提供生产特定产品的最有效途径的指南 果蝇基因组的改变。此外，结果将决定 考虑开展大规模淘汰赛项目是否可行 果蝇，以及这样一个项目的时间规模是多少。最后， 果蝇基因打靶的有效方法的可用性将有助于 使用果蝇作为了解人类疾病生物学的模型。