Core--Microarray and bioinformatics

核心--微阵列与生物信息学

• 批准号: 6563987
• 负责人: Mark Kunkel
• 金额: $ 29.68万
• 依托单位: UNIVERSITY OF ARIZONA
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-01-01 至 2002-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6563987
• 关键词: antineoplastics; biomedical facility; informatics; microarray technology; neoplasm /cancer genetics

Description (provided by applicant) The objective of the DNA Microarray and Bioinformatics Core Service is to use DNA microarray technology to rapidly and comprehensively determine the effects of anticancer agents on the patterns of gene expression in small samples of human minor cells, and to compare these patterns with reference patterns of gene expression that are produced by the same drugs in model in vitro and in vivo systems, cell culture and human tumor xenografts. The hypothesis upon which this approach is based is that the pattern of changes in gene expression can be used as a surrogate endpoint for 4mg effects; a reference pattern will be derived from the patterns of changes in gene expression which are produced in the model systems. The Core Service will use state of the art DN microarray equipment that is part of an established microarray facility at the Arizona Cancer Center. This includes all the necessary equipment for arraying, hybridizing and reading human gene chips; bacterial clone libraries for the production of microarray probes; and equipment for automating the PCR-based preparation of those probes. In addition, the protocols for the proposed work have been implemented and refined at the Arizona Cancer Center. The Bioinformatics support for this process will use current generation computing and communication technologies to store, manage, integrate and analyze the data from the microarray analyses.

描述（由申请人提供） DNA 微阵列和生物信息学核心服务的目标是利用 DNA微阵列技术可快速、全面地确定效果 抗癌药物对小样本基因表达模式的影响 人类小细胞，并将这些模式与参考模式进行比较 相同药物在体外和体内模型中产生的基因表达 体内系统、细胞培养和人类肿瘤异种移植物。假设基于 这种方法的基础是基因表达的变化模式 可用作 4mg 效果的替代终点；参考模式将 源自产生的基因表达变化模式 在模型系统中。核心服务将使用最先进的 DN 微阵列 设备是亚利桑那州已建立的微阵列设施的一部分 癌症中心。这包括排列所需的所有设备， 杂交和读取人类基因芯片；细菌克隆库 微阵列探针的生产；以及用于自动化基于 PCR 的设备 准备这些探针。此外，拟议工作的协议 已在亚利桑那州癌症中心实施和完善。这 该过程的生物信息学支持将使用当前一代计算 和通信技术来存储、管理、集成和分析 来自微阵列分析的数据。