Pilot--Microscale techniques for assessment of drug effects

中试——药物效应评估的微尺度技术

• 批准号: 6563984
• 负责人: Mark Kunkel
• 金额: $ 29.68万
• 依托单位: UNIVERSITY OF ARIZONA
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-01-01 至 2002-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6563984
• 关键词: antineoplastics; biomedical facility; drug screening /evaluation; fluidity; fluorimetry; immunochemistry; microarray technology; miniature biomedical equipment; surface property; technology /technique development

Description (provided by applicant) The objectives of this pilot project are to apply recent advances in the technologies of microfluidics, polymer surface chemistry and laser-scanning fluorometry to the task of simultaneously measuring the status of multiple protein1enzyme molecular targets in small clinical samples. The application of advanced technologies to the production of antibody arrays, and the technologies and protocols for executing the assays will allow straight-forward application of standard biochemical and immunological techniques. The goals of the project are 1) to miniaturize classical techniques of protein immunochemistry, through the modification and adaptation of established immunological methods of sandwich antibody assay and the use of liquid handling and laser-fluorometric technologies adapted from DNA microarray technology, and 2) to develop methods to allow these assays to be multiplexed so that several different endpoints can be analyzed in a single patient sample.

描述（由申请人提供） 该试点项目的目标是应用该领域的最新进展 微流体、聚合物表面化学和激光扫描技术 荧光测定法用于同时测量多个状态的任务 小临床样本中的蛋白质1酶分子靶点。应用 抗体阵列生产的先进技术，以及 执行检测的技术和协议将允许 标准生化和免疫学的直接应用 技术。该项目的目标是 1) 小型化经典 蛋白质免疫化学技术，通过修改和改编 已建立的夹心抗体测定的免疫学方法及其使用 源自 DNA 的液体处理和激光荧光技术 微阵列技术，以及 2) 开发方法以允许这些测定 多路复用，以便可以在单个端点中分析多个不同的端点 患者样本。