CORE--ADVANCED IMAGING SUPPORT

核心——高级成像支持

• 批准号: 6410370
• 负责人: Michael Kashgarian
• 金额: $ 14.1万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-12-01 至 2001-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6410370
• 关键词: bioimaging /biomedical imaging; biomedical facility; confocal scanning microscopy; digital imaging; electron microscopy; fluorescence microscopy; histopathology; imaging /visualization /scanning; immunocytochemistry

Description: (Abstract of the application) The major goal of the imaging core is to facilitate the use of quantitative and qualitative imaging techniques in the analysis of protein interactions at the cellular and molecular level. A wide range of imaging techniques applicable to the study of membrane and cytoskeletal proteins in cells, tissues, whole organs are provided. In addition, the utilization of transgenic technology offers new opportunities to study the phenotypic changes at the integrated whole animal level. New technologies are now available for greater resolution of localization of specific target proteins as well as permitting vital microscopy where dynamic changes can be identified. Paraffin section light microscopy, still the gold standard for the examination of structural phenotypic changes, has been expanded to include localization of specific proteins using immunohistochemistry and of specific mRNAs using in situ hybridization and in situ reverse transcription. Confocal microscopy can be applied to tissues where analyses of changes in intracellular ion concentration can be assessed at the tissue level using ion sensitive fluorochromes resulting in an expansion of the capabilities of video imaging techniques which are applied at the cellular level. The utilization of electron microscopic immunocytochemical techniques has been improved for greater accuracy in localization using immunogold and in situ hybridization techniques. The value of these techniques is enhanced by the availability of digital image analysis systems that permit quantitative morphometric measurements and three-dimensional reconstruction and visualization. These techniques are extremely useful in cellular pathobiology but are not easily accessible to investigators and research trainees without previous training or experience. In addition the selection of the proper technique or combination of techniques requires experience in interpretation and knowledge of the limitations of the method. The imaging core will provide expertise and assistance in the utilization of light, immunofluorescence, confocal, and electron microscopy with particular emphasis on phenotypic and pathologic analysis of transgenic models, immunocytochemistry, vital microscopy and image analysis. The facility will give access to these specialized techniques and to the equipment necessary to apply them and will form a focal point for collaboration between members of the program project. Specifically, the core will provide the facilities, service and education necessary for the efficient application of imaging techniques tailored to the individual research objectives of the various members of the program project.

描述：（应用的摘要）成像核心的主要目标是促进在细胞和分子水平的蛋白质相互作用分析中使用定量和定性成像技术。提供了多种成像技术，可用于研究细胞，组织，整个器官中膜和细胞骨架蛋白的研究。此外，转基因技术的利用为研究整个动物层面的表型变化提供了新的机会。现在可以使用新技术来更好地解析特定靶蛋白的定位，并允许在可以识别动态变化的重要显微镜下进行重要的显微镜检查。石蜡截面光学显微镜仍然是检查结构表型变化的金标准，已扩展，包括使用免疫组织化学和使用原位杂交和原位逆转录的特定蛋白质定位。共聚焦显微镜可以应用于组织，其中可以使用离子灵敏荧光染料在组织水平上评估细胞内离子浓度变化，从而在组织水平上评估，从而扩大了在细胞水平上应用的视频成像技术的能力。电子显微镜免疫细胞化学技术的利用已得到提高，以提高使用免疫元和原位杂交技术的定位。这些技术的价值通过允许定量形态测量以及三维重建和可视化的数字图像分析系统的可用性增强。这些技术在细胞病理生物学中非常有用，但没有以前的培训或经验，研究人员和研究受训者不容易获得。另外，选择适当的技术或技术组合还需要在解释和了解该方法局限性方面的经验。成像核心将在利用光，免疫荧光，共聚焦和电子显微镜的利用方面提供专业知识和帮助，并特别强调转基因模型，免疫细胞化学，重要显微镜和图像分析的表型和病理分析。该设施将访问这些专门的技术以及应用它们所需的设备，并将成为计划项目成员之间协作的焦点。具体而言，核心将提供针对该计划项目各个成员的个人研究目标量身定制成像技术所需的设施，服务和教育。