CORE--MICROSCOPY AND IMAGING CENTER

核心——显微成像中心

• 批准号: 6725904
• 负责人: Michael Kashgarian
• 金额: $ 19.74万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-09-30 至 2008-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6725904
• 关键词: bioimaging /biomedical imaging; biomedical facility; computer program /software; confocal scanning microscopy; digital imaging; electron microscopy; histochemistry /cytochemistry; imaging /visualization /scanning; immunofluorescence technique; in situ hybridization; morphometry; renal tubular transport; tissue /cell preparation; video microscopy

The major goal of the Cell Biology Core is to facilitate the use of quantitative qualitative imaging techniques and in the analysis of transport processes at the cellular and molecular level. The range of imaging techniques which are applicable to the study of cells, tissues, whole organs and in wild type, transgenic and mutant animals have been greatly expanded. New technologies are now available for greater resolution of localization of specific target proteins as well as permitting vital microscopy where dynamic changes can be identified. These include immunocytochemistry, confocal microscopy, electron microscopy, in situ hybridization, uunbiased stereological morphometry, and vital microscopy. These techniques are extremely useful in cell biology and physiology but are not easily accessible to investigators without previous training or experience. In addition, the selection of the proper technique or combination of techniques requires experience in interpetation and knowledge of the limitations of the method. The core will provide expertise and assistance in the utilization of light, immunofluorescence, confocal, and electron microscopy with particular emphasis on phenotypic and pathologic analysis of transgenic models, immunocytochemistry, vital microscopy and image analysis. The facility will give access to these specialized techniques and to the equipment necessary to apply them and will form a focal point for collaboration between members of the program project. Specifically, the core will provide the facilities, service and education necessary for the efficient application of imaging techniques tailored to the individual research objectives of the various members.

细胞生物学核心的主要目的是促进定量定性成像技术的使用以及在细胞和分子水平的传输过程中分析。适用于细胞，组织，整个器官以及野生型，转基因和突变动物的成像技术的范围已大大扩展。现在可以使用新技术来更好地解析特定靶蛋白的定位，并允许在可以识别动态变化的重要显微镜下进行重要的显微镜检查。这些包括免疫细胞化学，共聚焦显微镜，电子显微镜，原位杂交，Uunbiabiabialed立体形态计量学和重要显微镜。这些技术在细胞生物学和生理学中非常有用，但是如果没有以前的培训或经验，研究人员就不容易获得。此外，选择适当的技术或技术组合需要插入和了解局限性的经验 方法。核心将在光，免疫荧光，共聚焦和电子显微镜的利用中提供专业知识和帮助，并特别强调转基因模型，免疫细胞化学，重要显微镜和图像分析的表型和病理分析。该设施将访问这些专门的技术以及应用它们所需的设备，并将成为计划项目成员之间协作的焦点。具体而言，核心将为有效地应用针对各个成员的个人研究目标量身定制成像技术所需的设施，服务和教育。