Core-B-Specialized imaging

核心-B-专业成像

• 批准号: 6910147
• 负责人: Michael Kashgarian
• 金额: $ 7.16万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-06-17 至 2009-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6910147
• 关键词: atomic force microscopy; bioimaging /biomedical imaging; biological transport; biomedical facility; confocal scanning microscopy; electron microscopy; hypoxia; immunocytochemistry; immunofluorescence technique; light microscopy; phenotype

The major goal of the Specialized Imaging Core is to facilitate the use of quantitative and qualitative imaging techniques in the analysis of transport physiology at the cellular and molecular level. The range of imaging techniques which are applicable to the study of transport systems in cells, tissues, whole organs and in the intact organism have been greatly expanded. New technologies are now available for greater resolution of localization of specific target proteins as well as permitting vital microscopy where dynamic changes can be identified. These include immunocytochemistry, confocal microscopy, electron microscopy, in situ hybridization, morphometry, atomic force microscopy and vital microscopy. These techniques are extremely useful in cell physiology and biology but are not easily accessible to investigators without previous training or experience. In addition, the selection of the proper technique or combination of techniques requires experience in interpretation and knowledge of the limitations of the method. The Imaging core will provide expertise and assistance in the utilization of light, immunofluorescence, confocal, atomic force and electron microscopy with particular emphasis on phenotypic and pathologic analysis of transgenic models, immunocytochemistry, vital microscopy and image analysis. The facility will give access to these specialized techniques and to the equipment necessary to apply them and will form a focal point for collaboration between members of the program project. Specifically, the core will provide the facilities, service and education necessary for the efficient application of imaging techniques tailored to the individual research objectives of the various members of the program project.

专业成像核心的主要目的是促进在细胞和分子水平的传输生理学分析中使用定量和定性成像技术。适用于细胞，组织，整个器官以及完整生物体中运输系统的成像技术的范围已大大扩展。现在可以使用新技术来更好地解决特定目标的定位 蛋白质以及允许可以鉴定动态变化的重要显微镜。这些包括免疫细胞化学，共聚焦显微镜，电子显微镜，原位杂交，形态计量学，原子力显微镜和重要显微镜。这些技术在细胞生理和生物学中非常有用，但是如果没有以前的培训或经验，研究人员就不容易获得。此外，选择适当的技术或技术组合需要解释和了解局限性的经验 方法。成像核心将在利用光，免疫荧光，共聚焦，原子力和电子显微镜的利用方面提供专业知识和辅助，并特别强调转基因模型，免疫细胞化学，生命显微镜和图像分析的表型和病理分析。该设施将访问这些专门的技术以及应用它们所需的设备，并将成为计划项目成员之间协作的焦点。具体而言，核心将提供针对该计划项目各个成员的个人研究目标量身定制成像技术所需的设施，服务和教育。