CORE--ADVANCED IMAGING SUPPORT

核心——高级成像支持

• 批准号: 6564381
• 负责人: Michael Kashgarian
• 金额: $ 14.1万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-12-01 至 2002-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6564381
• 关键词: bioimaging /biomedical imaging; biomedical facility; confocal scanning microscopy; digital imaging; electron microscopy; fluorescence microscopy; histopathology; imaging /visualization /scanning; immunocytochemistry

Description: (Abstract of the application) The major goal of the imaging core is to facilitate the use of quantitative and qualitative imaging techniques in the analysis of protein interactions at the cellular and molecular level. A wide range of imaging techniques applicable to the study of membrane and cytoskeletal proteins in cells, tissues, whole organs are provided. In addition, the utilization of transgenic technology offers new opportunities to study the phenotypic changes at the integrated whole animal level. New technologies are now available for greater resolution of localization of specific target proteins as well as permitting vital microscopy where dynamic changes can be identified. Paraffin section light microscopy, still the gold standard for the examination of structural phenotypic changes, has been expanded to include localization of specific proteins using immunohistochemistry and of specific mRNAs using in situ hybridization and in situ reverse transcription. Confocal microscopy can be applied to tissues where analyses of changes in intracellular ion concentration can be assessed at the tissue level using ion sensitive fluorochromes resulting in an expansion of the capabilities of video imaging techniques which are applied at the cellular level. The utilization of electron microscopic immunocytochemical techniques has been improved for greater accuracy in localization using immunogold and in situ hybridization techniques. The value of these techniques is enhanced by the availability of digital image analysis systems that permit quantitative morphometric measurements and three-dimensional reconstruction and visualization. These techniques are extremely useful in cellular pathobiology but are not easily accessible to investigators and research trainees without previous training or experience. In addition the selection of the proper technique or combination of techniques requires experience in interpretation and knowledge of the limitations of the method. The imaging core will provide expertise and assistance in the utilization of light, immunofluorescence, confocal, and electron microscopy with particular emphasis on phenotypic and pathologic analysis of transgenic models, immunocytochemistry, vital microscopy and image analysis. The facility will give access to these specialized techniques and to the equipment necessary to apply them and will form a focal point for collaboration between members of the program project. Specifically, the core will provide the facilities, service and education necessary for the efficient application of imaging techniques tailored to the individual research objectives of the various members of the program project.

描述：（申请摘要）成像核心的主要目标是促进定量和定性成像技术在细胞和分子水平上蛋白质相互作用分析中的使用。提供了适用于细胞、组织、整个器官中膜和细胞骨架蛋白研究的广泛成像技术。此外，转基因技术的利用为研究整体动物水平上的表型变化提供了新的机会。现在新技术可以提高特定靶蛋白定位的分辨率，并允许使用重要的显微镜来识别动态变化。石蜡切片光学显微镜仍然是检查结构表型变化的金标准，现已扩展到包括使用免疫组织化学定位特定蛋白质以及使用原位杂交和原位逆转录定位特定 mRNA。共焦显微镜可应用于组织，其中可以使用离子敏感荧光染料在组织水平评估细胞内离子浓度变化的分析，从而扩展在细胞水平应用的视频成像技术的能力。电子显微镜免疫细胞化学技术的利用得到了改进，使用免疫金和原位杂交技术进行定位的准确性更高。数字图像分析系统的可用性增强了这些技术的价值，该系统允许定量形态测量以及三维重建和可视化。这些技术在细胞病理学中非常有用，但对于没有接受过培训或经验的研究人员和研究实习生来说不容易使用。此外，选择适当的技术或技术组合需要解释经验和了解该方法的局限性。成像核心将在光、免疫荧光、共聚焦和电子显微镜的利用方面提供专业知识和帮助，特别强调转基因模型的表型和病理分析、免疫细胞化学、活体显微镜和图像分析。该设施将提供这些专门技术以及应用这些技术所需的设备，并将成为该计划项目成员之间合作的焦点。具体来说，核心将提供有效应用成像技术所需的设施、服务和教育，这些技术是根据该计划项目各成员的个人研究目标量身定制的。