LM/EM IMAGING

LM/EM 成像

基本信息

批准号：
8364959
负责人：
Robert B. Wysolmerski
金额：
$ 21.76万
依托单位：
WEST VIRGINIA UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2011
资助国家：
美国
起止时间：
2011-07-01 至 2012-06-30
项目状态：
已结题

项目摘要

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The Light and Electron Microscopic (LM/EM) Imaging Core Facility provides image acquisition and analysis services on a fee-per-use basis. The facility contains a broad range of instruments that allow visualization of fixed tissue, in vitro cell and tissue preparations and in vivo imaging. Broadly, instrumentation can be divided into standard fluorescence-based capabilities such as upright microscopes with digital camera image capture and confocal microscopy that we term linear optical microscopy (LOM) to contrast with non-linear optical microscopy (NLOM) methods that are based on scanning with fast-pulsed lasers, such as multiphoton microscopy. The facility is staffed with two doctorate-trained managers to facilitate expert consultation for experimental design, training on use of equipment, and efficient access to equipment for data collection. The NLOM system is designed as a technology development core through collaboration with a faculty member and trainees in physics to devise new approaches to biomedical imaging. This facility is unique within the state of West Virginia, and the NLOM capabilities are available in very few centers nationwide. Support to further build and sustain NLOM is the focus of our request in this application. The LM/EM Core is directed by a faculty member, Dr. Robert Wysolmerski, who has substantial experience with both LM and EM imaging modalities and who ran an EM core prior to joining WVU. The co-director, Dr. Feruz Ganikhanov, has extensive experience in laser physics and is tasked to develop new imaging applications for NLOM in consultation or collaboration with biomedical scientists. He oversees a laboratory manager who also has doctoral training in laser physics and who assists investigators during data collection and analysis. Because we request support primarily for these NLOM capabilities, we devote much of this section to their description. Newly constructed laboratory space in the Erma Byrd Biomedical Research Building has been allocated for the LM/EM Imaging Core. The third floor of that building houses the NLOM and one confocal microscope; the bulk of the remaining equipment is housed in an integrated facility on the second floor.

该子项目是利用资源的众多研究子项目之一由 NIH/NCRR 资助的中心拨款提供。子项目的主要支持并且子项目的首席研究员可能是由其他来源提供的，包括其他 NIH 来源。子项目可能列出的总成本代表子项目使用的中心基础设施的估计数量， NCRR 赠款不直接向子项目或子项目工作人员提供资金。光学和电子显微镜 (LM/EM) 成像核心设施按每次使用收费提供图像采集和分析服务。该设施包含各种仪器，可实现固定组织、体外细胞和组织制剂以及体内成像的可视化。从广义上讲，仪器可分为基于标准荧光的功能，例如具有数码相机图像捕获功能的正置显微镜和共焦显微镜，我们将其称为线性光学显微镜（LOM），以与基于扫描的非线性光学显微镜（NLOM）方法进行对比使用快速脉冲激光，例如多光子显微镜。该设施配备了两名受过博士学位的管理人员，以促进实验设计的专家咨询、设备使用培训以及有效使用数据收集设备。 NLOM 系统被设计为技术开发核心，通过与物理领域的教员和实习生合作，设计生物医学成像的新方法。该设施在西弗吉尼亚州是独一无二的，并且全国很少有中心提供 NLOM 功能。支持进一步构建和维持 NLOM 是我们在此应用程序中请求的重点。 LM/EM 核心由教员 Robert Wysolmerski 博士指导，他在 LM 和 EM 成像模式方面拥有丰富的经验，并且在加入 WVU 之前运行过 EM 核心。联合主任 Feruz Ganikhanov 博士在激光物理学方面拥有丰富的经验，其任务是与生物医学科学家协商或合作，为 NLOM 开发新的成像应用。他负责监督一名实验室经理，该经理也接受过激光物理学博士学位培训，并在数据收集和分析过程中协助研究人员。因为我们主要请求支持这些 NLOM 功能，所以本节的大部分内容都用于描述它们。 Erma Byrd 生物医学研究大楼新建的实验室空间已分配给 LM/EM 成像核心。该大楼的三楼设有 NLOM 和一台共焦显微镜；其余大部分设备均安置在二楼的综合设施内。