Core--Imaging

核心--影像

• 批准号: 7429101
• 负责人: Keigi Fujiwara
• 金额: $ 12.4万
• 依托单位: UNIVERSITY OF ROCHESTER
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-08-01 至 2010-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7429101
• 关键词: atherosclerosis; bioimaging /biomedical imaging; biomedical facility; computer simulation; confocal scanning microscopy; fluorescence resonance energy transfer; image processing; imaging /visualization /scanning; immunofluorescence technique; inflammation; phase contrast microscopy

At present, the Imaging Core of the Center for Cardiovascular Research houses one Olympus laser scanning confocal microscope (Fluoview FV300) equipped with Krypton, Argon and He/Ne lasers for confocal microscopy and Mercury and Halogen lamps for epifluorescence and DIC/phase contrast/bright field microscopy, respectively. The confocal unit is mounted on an inverted Olympus microscope (1X70) with a universal high NA condenser. For capturing non-confocal images, a Spot CCD camera is used. This confocal microscope will be used for the proposed PPG projects. There is an up-right Olympus microscope (BX51) for epifluorescence, phase contrast, and DIC observations. The scope is also equipped with a Spot CCD camera. This scope is used for routine immunofluorescence observations, especially for en face analyses of immunostained endothelial tissue. Specimens for immunofluorescence microscopy are prepared by individual investigators. Technical suggestions and advice are given to them, such as fixation and staining conditions, blocking, and mounting. Data acquisition is done with the help of the Core Director and an assistant using a TV monitor. Those who wish to use the scopes by themselves are given instructions by the Core Director. MCID Elite 6.0 (Imaging Research Inc.) is available for the studies proposed in this application. The software is capable of performing various forms of image analyses such as image enhancement, image processing and editing, and 3D reconstruction. It has also functions that are quantitative such as profiling (vertical, horizontal, rotatable, and user-traced lane definition), stereology for unbiased morphological measurements and quantification, and grain counting. The grain counting mode is applicable to cells, grains, organelles, and other discrete objects. Valid targets are discriminated from background based on their intensity, color and spatial criteria.

目前，心血管研究中心的成像核心设有一个Olympus激光扫描 配备了K，Argon和He/Ne激光器的共聚焦显微镜（Fluoview FV300）用于共聚焦显微镜 分别用于汞荧光和DIC/相对比/明亮场显微镜的汞和卤素灯。 共聚焦单元安装在带有通用高Na冷凝器的倒置奥林匹克显微镜上（1x70）。为了 捕获非焦点图像，使用了点CCD摄像头。该共聚焦显微镜将用于 拟议的PPG项目。 有一个高右右眼显微镜（BX51）用于落荧光，相对比和DIC 观察。范围还配备了点CCD摄像头。此范围用于例行 免疫荧光观察，尤其是针对免疫染色的内皮组织的分析。 免疫荧光显微镜的标本由个别研究者制备。技术的 提出建议和建议，例如固定和染色条件，阻塞和安装。数据 收购是在使用电视显示器的核心主管和助手的帮助下完成的。那些想使用的人 核心主管本身会指示这些范围。 MCID Elite 6.0（Imaging Research Inc.）可用于本申请中提出的研究。这 软件能够执行各种形式的图像分析，例如图像增强，图像处理 和编辑，以及3D重建。它还具有定量的功能，例如分析（垂直，水平， 可旋转和用户跟踪的车道定义），无偏形形态测量和量化的立体学， 和谷物计数。谷物计数模式适用于细胞，谷物，细胞器和其他离散物体。 根据其强度，颜色和空间标准，有效目标与背景区分开。