LASER CAPTURE MICROSCOPE

激光捕获显微镜

• 批准号: 2775662
• 负责人: DAN MERCOLA
• 金额: $ 8.67万
• 依托单位: SIDNEY KIMMEL CANCER CENTER
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-04-15 至 2000-04-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2775662
• 关键词: bioimaging /biomedical imaging; biomedical equipment; biomedical equipment purchase; computer network; computer program /software; confocal scanning microscopy; digital imaging; dissection; lasers; tissue resource /registry

The Sidney Kimmel Cancer Center is a not-for-profit basic research institute founded in 1991 specializing in cancer research with an emphasis on the development of gene therapy, especially immuno-gene therapy, antisense gene, and related therapies for the treatment of glioblastoma and prostate, breast, and colon carcinomas. The center has grown rapidly to 19 principal investigators and over 90 support personal. All research programs are supported in part by one or more competitively awarded research grants mostly from the USPH Service. A major core facility, the Tumor Registry, is being developed for the fresh and cryopreserved normal and tumor specimens for genetic analysis. The registry is unique in that a program of rapid acquisition and cryopreservation has been developed including quality control and characterization of all samples. Investigators of a number of NIH- supported programs noted that the goals of their programs would be greatly facilitated by the availability of the newly developed laser capture microdissection instrument such as the Arcturus PixCellTM. An integrated program is proposed to provide this instrument as part of the Tumor Registry. Moreover, the instrument will be an on-line facility via the institutional server to all users in house and in the surrounding community. Finally, the instrument will be adapted to carry out two- and three-dimensional morphometric analysis and quantitative analysis of tissue sections labeled by histochemical methods, immunohistochemical methods or in situ hybridization methods. Labeling also may be used to electronically identify by predefined criteria all lesions for microdissection. Thus, a highly versatile, unique and generally available community facility is proposed for multigene and morphometric analysis of human tumor specimens.

悉尼金梅尔癌症中心是一家非营利性基础研究机构，成立于1991年，专门从事癌症研究，重点发展基因治疗，特别是免疫基因治疗、反义基因以及治疗胶质母细胞瘤和癌症的相关疗法。前列腺癌、乳腺癌和结肠癌。 该中心已迅速发展到 19 名主要研究人员和 90 多名支持人员。 所有研究项目都部分由一项或多项竞争性授予的研究资助提供支持，这些资助主要来自 USPH 服务。 一个主要的核心设施——肿瘤登记处，正在开发中，用于新鲜和冷冻保存的正常和肿瘤标本进行基因分析。该注册中心的独特之处在于开发了快速采集和冷冻保存程序，包括所有样品的质量控制和表征。 NIH 支持的许多项目的研究人员指出，新开发的激光捕获显微切割仪器（例如 Arcturus PixCellTM）的可用性将极大地促进他们项目的目标的实现。 建议实施一项综合计划来提供该工具作为肿瘤登记的一部分。 此外，该仪器将通过机构服务器成为内部和周边社区所有用户的在线设施。最后，该仪器将适用于对组织化学方法、免疫组织化学方法或原位杂交方法标记的组织切片进行二维和三维形态分析和定量分析。标记还可以用于通过预定义的标准以电子方式识别所有用于显微切割的病变。 因此，提出了一种高度通用、独特且普遍可用的社区设施，用于人类肿瘤标本的多基因和形态分析。