BIOMOLECULAR IMAGING SYSTEM

生物分子成像系统

• 批准号: 2803514
• 负责人: THOMAS COSTIN REGISTER
• 金额: $ 8.45万
• 依托单位: WAKE FOREST UNIVERSITY HEALTH SCIENCES
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-04-15 至 2001-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2803514
• 关键词: bioimaging /biomedical imaging; biomedical equipment; biomedical equipment purchase; densitometry; digital imaging; molecular dynamics

The purpose of this shared instrument grant application is to obtain NCRR funds to purchase an analytical imaging system to be shared by a group of NIH funded investigators at the Comparative Medicine Clinical Research Center of the Wake Forest University School of Medicine. The Major User Group is supported by 2 Program Projects and 2 RO1 grants from 4 separate NIH institutes. These investigators have critical needs for sensitive, quantitative, molecular analyses in order to effective advance their research. The instrumentation requested is a Molecular Dynamics STORM 860 PDSI Analytical Imaging System, a unique system which has capability of storage phosphor autoradiography (phosphorimaging), direct fluorescence analysis, and chemifluorescence analysis. These characteristics facilitate a broad range of analytical assays useful in nucleic acid and protein analysis. The instrument has a wide, linear dynamic range useful for quantitative data collection at any exposure intensity, eliminating the need for repeated scans and variable exposure times. Other features include high-resolution images, short scan times, large format capability, and powerful software to rapidly provide accurate data. No comparable equipment capable of performing any of these functions currently exists at this facility. The on site availability of the instrumentation will greatly expand the analytical capabilities of the Major User Group and will attract more users and applications once available. This system will enable us to carry out all of our analyses on site, which will increase investigator productivity and efficiency in the investigation of mechanisms involved in a variety of disease processes. The Section on Comparative Medicine of the Wake Forest University School of Medicine fully supports the proposed plan, and will contribute to the effort by providing space and continuing support for the maintenance of the instrumentation as necessary.

此共享仪器拨款申请的目的是获得 NCRR 资金来购买分析成像系统，由 NIH 资助的维克森林大学医学院比较医学临床研究中心的一组研究人员共享。主要用户组由来自 4 个独立 NIH 机构的 2 个计划项目和 2 个 RO1 拨款支持。这些研究人员迫切需要灵敏、定量的分子分析，以便有效推进他们的研究。所需的仪器是 Molecular Dynamics STORM 860 PDSI 分析成像系统，这是一个独特的系统，具有存储磷光放射自显影（磷光成像）、直接荧光分析和化学荧光分析的能力。这些特性有利于核酸和蛋白质分析中广泛的分析测定。该仪器具有宽广的线性动态范围，适用于任何曝光强度下的定量数据收集，无需重复扫描和可变曝光时间。其他功能包括高分辨率图像、短扫描时间、大格式功能以及可快速提供准确数据的强大软件。该设施目前不存在能够执行任何这些功能的类似设备。仪器的现场可用性将极大地扩展主要用户组的分析能力，并且一旦可用将吸引更多的用户和应用。该系统将使我们能够在现场进行所有分析，这将提高研究者在研究各种疾病过程所涉及的机制时的生产力和效率。维克森林大学医学院比较医学科完全支持拟议的计划，并将根据需要为仪器维护提供空间和持续支持，从而为这项工作做出贡献。