DYNAMIC NEURAL ORGANIZATION IN NEOCORTEX

新皮质的动态神经组织

• 批准号: 6393537
• 负责人: Jian-Young Wu
• 金额: $ 23.51万
• 依托单位: GEORGETOWN UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-09-15 至 2003-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6393537
• 关键词: bioimaging /biomedical imaging; brain cell; brain electrical activity; brain imaging /visualization /scanning; cell population study; evoked potentials; laboratory rat; microelectrodes; neocortex; neuroanatomy; stainings; voltage /patch clamp

The long-term objective of this study is to understand the local population interactions in neocortex, an important process for both normal cortical functioning and for neurological disorders such as epilepsy. Our approach is to examine the spatio-temporal patterns and cellular properties of population activity in intrinsic cortical circuits. We hypothesize that one basic form of population activation in neocortex is the dynamically organized multi-neuronal ensemble. In our previous study we found a local co-activation in adult cortical slices which is related to the activity patterns in the cortex. This kind of co- activation is dynamically organized, all-or-none population event with an asynchronized, low density of spikes. It could be evoked by a proper stimulation or spontaneously occurred as a propagating activation during 7 to 10 Hz oscillations. This proposal will utilize voltage-sensitive dye imaging and electrophysiological methods to further study the spatio-temporal dynamics and neuronal activities of this cortical population event. There are four specific aims. Aim 1 will examine the proper stimulation patterns which can evoke or interact with this activity, and explore whether this activity can commonly occur in different cortical areas. Aim 2 will study the network and cellular mechanisms which sustain this co-activation. Aim 3 will study propagation of this activity and the interactions with the cortical columnar organization. Aim 4 will study the neuronal activity within this dynamically organized population event. This study will increase our knowledge about the cortical population activities related to the input pattern and intrinsic activities in cortex during normal and pathological conditions.

这项研究的长期目标是了解新皮质中当地人群的相互作用，这是正常皮质功能和癫痫等神经系统疾病的重要过程。 我们的方法是检查内在皮质回路中群体活动的时空模式和细胞特性。 我们假设新皮质群体激活的一种基本形式是动态组织的多神经元集合。 在我们之前的研究中，我们发现成人皮质切片中存在局部共激活，这与皮质中的活动模式有关。 这种共激活是动态组织的、全有或全无的群体事件，具有异步、低密度的尖峰。 它可以通过适当的刺激引起，也可以在 7 至 10 Hz 振荡期间作为传播激活自发发生。 该提案将利用电压敏感染料成像和电生理学方法来进一步研究这一皮质群体事件的时空动力学和神经元活动。 有四个具体目标。 目标 1 将检查可以引起该活动或与该活动相互作用的适当刺激模式，并探讨该活动是否可以普遍发生在不同的皮质区域。 目标 2 将研究维持这种共同激活的网络和细胞机制。 目标 3 将研究这种活动的传播以及与皮质柱状组织的相互作用。 目标 4 将研究这个动态组织的群体事件中的神经元活动。这项研究将增加我们对正常和病理条件下皮层输入模式和内在活动相关的皮层群体活动的了解。