MULTIPHOTON SCANNING LASER FLUORESCENCE MICROSCOPE

多光子扫描激光荧光显微镜

• 批准号: 6291330
• 负责人: WILLIAM M ROBERTS
• 金额: $ 50万
• 依托单位: UNIVERSITY OF OREGON
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-04-01 至 2002-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6291330
• 关键词: biomedical equipment purchase; fluorescence microscopy

This Shared Instrumentation Grant proposal requests funds to purchase a Zeiss LSM 510 NLO multiphoton laser scanning microscope, to be installed in the Bio-Optics Laboratory at the University of Oregon. This microscope will be used to advance ongoing NIH supported research in numerous laboratories in the Institutes of Neuroscience and Molecular Biology at the University of Oregon, including six major users who have described specific projects that will be greatly facilitated by the availability of a multi-photon fluorescence microscope. The two main benefits offered by this new technology are (1) the capability performing time-lapse microscopy on living specimens with greatly reduced photodynamic damage or bleaching of fluorescent labels and (2) the ability to obtain crisp images at depths of 100 mu m or more into a specimen. Five of the major users study the developmental biology of zebrafish (Judith Eisen, Charles Kimmel and John Postlethwait), Drosophila (Chris Doe), tunicates (John Postlethwait) and C. elegans (Bruce Bowerman). One user (William Roberts) studies synaptic physiology and calcium signaling in sensory receptors in the frog ear. The microscope will be available as a shared facility for use by life scientists at the University of Oregon.

这项共享的仪器赠款提案要求资金购买Zeiss LSM 510 NLO Multiphoton激光扫描显微镜，该显微镜将安装在俄勒冈大学的生物学实验室中。该显微镜将用于推进俄勒冈大学神经科学和分子生物学研究所的众多实验室中正在进行的NIH支持研究，其中包括六个主要用户，这些主要用户描述了特定的项目，这些项目将通过多光子的可用性极大地促进。荧光显微镜。这项新技术提供的两个主要好处是（1）在活体标本上执行延时显微镜的功能，其光动力损害大大降低或荧光标签的漂白以及（2）能够在100 mu M或100 mu m或100 mu m或更多地进入标本。五名主要用户研究斑马鱼（朱迪思·艾森，查尔斯·金梅尔和约翰·波斯特维特），果蝇（Chris doe），束缚犬（John Postlethwait）和C. elegrans（Bruce Bowerman）的发育生物学。一位用户（威廉·罗伯茨（William Roberts））研究青蛙耳朵中感觉受体中的突触生理和钙信号传导。显微镜将作为共享设施提供，供俄勒冈大学的生活科学家使用。