Migratory Properties of CD8 T Cells

CD8 T 细胞的迁移特性

• 批准号: 6876864
• 负责人: ULRICH H VON ANDRIAN
• 金额: $ 43.92万
• 依托单位: IMMUNE DISEASE INSTITUTE, INC.
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-04-01 至 2006-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6876864
• 关键词: T lymphocyte; biomedical equipment purchase; bone marrow; cell migration; confocal scanning microscopy; dendritic cells; fluorescence microscopy

DESCRIPTION (provided by applicant): Funds are requested for the purchase of a dedicated system to perform multiphoton intravital microscopy (MPIVM), which will be installed as a core instrument in the CBR Institute for Biomedical Research. The new MPIVM system will constitute an expansion of the Institute's existing multiphoton/confocal microscopy core facility. User demand for access to the established facility far exceeds its capacity, which has caused month-long waiting lists and severely restricts investigators' ability to make progress in NIH-funded research projects. The most rapid growth in usage of the existing setup has been in the area of intravital microscopy whereby three-dimensional time-lapse movies are generated to study intra- and extravascular events in lymph nodes, bone marrow and the brain of anesthetized mice. While investigator-initiated preliminary work in this area has been remarkably productive, possibility for further progress is limited due to lack of access and also because the current core facility was designed to exhibit maximum versatility and is not specifically optimized for MPIVM imaging. The proposed new system will thus meet an urgent need of Major Users who will use MPIVM to pursue the following NIH-funded research projects: 1.) Imaging of antigen trafficking in vivo (M.C. Carroll); 2.) Visualization of immune cell traffic in bone marrow (IB. Mazo); 3.) Visualization of MHC class II transport in dendritic cells (H. Ploegh); 4.) T cell migration and differentiation in lymph nodes (U.H. von Andrian); and 5.) Assessment of blood-brain barrier function in vivo (D.D. Wagner). The CBR Institute for Biomedical Research has committed institutional funds for construction/renovation of a dedicated room and to purchase a new Olympus BX51 fluorescence microscope and a vibration-isolated table system, which are integral components of the MPIVM setup. CBR funds will also support a full-time Optical Imaging Facility Manager and a stand-alone workstation for image analysis and data processing. Funds are requested to procure a MaiTai broadband mode-locked Ti:Sapphire laser (Spectra-Physics) and a multiphoton-optimized Radiance 2100 laser scanning system from Bio-Rad.

描述（由申请人提供）：需要资金购买专用系统来执行多光子活体显微镜检查（MPIVM），该系统将作为核心仪器安装在 CBR 生物医学研究所。新的 MPIVM 系统将构成研究所现有多光子/共焦显微镜核心设施的扩展。用户对使用已建立设施的需求远远超过其容量，这导致了长达一个月的等待名单，并严重限制了研究人员在 NIH 资助的研究项目中取得进展的能力。现有装置的使用增长最快的是活体显微镜领域，通过生成三维延时电影来研究麻醉小鼠的淋巴结、骨髓和大脑的血管内和血管外事件。虽然研究人员在这一领域发起的初步工作非常富有成效，但由于缺乏访问权限，而且当前的核心设施旨在展示最大的多功能性，并且没有专门针对 MPIVM 成像进行优化，进一步取得进展的可能性受到限制。因此，拟议的新系统将满足主要用户的迫切需求，他们将使用 MPIVM 开展以下 NIH 资助的研究项目： 1.) 体内抗原运输成像（M.C. Carroll）； 2.) 骨髓中免疫细胞运输的可视化（IB. Mazo）； 3.) 树突状细胞中 MHC II 类转运的可视化 (H. Ploegh)； 4.) T细胞在淋巴结中的迁移和分化（U.H. von Andrian）； 5.) 体内血脑屏障功能评估（D.D. Wagner）。 CBR 生物医学研究所已投入机构资金用于建造/翻新一个专用房间，并购买一台新的奥林巴斯 BX51 荧光显微镜和一个隔振台系统，这些都是 MPIVM 设置的组成部分。 CBR 资金还将支持一名全职光学成像设施经理和一个用于图像分析和数据处理的独立工作站。需要资金从 Bio-Rad 采购一台 MaiTai 宽带锁模钛宝石激光器（Spectra-Physics）和一台多光子优化 Radiance 2100 激光扫描系统。