Laser Scanning Confocal Microscope

激光扫描共焦显微镜

• 批准号: 6878328
• 负责人: STEFAN HERLITZE
• 金额: $ 49.66万
• 依托单位: CASE WESTERN RESERVE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-04-15 至 2006-04-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6878328
• 关键词: biomedical equipment purchase; confocal scanning microscopy; fluorescence resonance energy transfer; neuroimaging

DESCRIPTION (provided by applicant): Funds are requested to purchase a laser scanning confocal microscope. This system, the Zeiss LSM 510 Meta coupled to an Axiovert 200 MOT, will replace a 10-year-old LSM 410 system with 232 registered users from 29 departments. The requested system will be housed and administered by the Neuroscience Imaging Center (NIC), the oldest imaging core facility at our University. The NIC employs a full-time technician with 8 years of experience operating the LSM 410 confocal microscope. Projects from 8 major and 9 minor users are described. These projects will benefit from the improved optics and detection system of the LSM 510 Meta, resulting in improved capture of fluorochrome emission, spectral separation, reduced phototoxicity and the ability to do fluorescence resonance energy transfer (FRET) measurements. Projects of the major users include: Ca 2+ channel trafficking in hippocampal slices or cultures of rat and mice (Dr. Herlitze), role and mechanical function of myosin II during cell locomotion in human cancer cells (Dr. Egelhoff), development of new mouse strains as genetic based tools for brain serotonergic neuron studies (Dr. Deneris), regulation of 5-HT-receptor targeting and trafficking (Dr. Roth), role of dynamin interacting proteins in the endocytotic cycle in Drosophila melanogaster (Dr. Robinson), role of endocytosis in EGF receptor trafficking (Dr. Carlin), regulation of postnatal cerebellar differentiation (Dr. Siegel) and axon regeneration across glial scar (Dr. Silver).

描述（由申请人提供）：需要资金购买激光扫描共焦显微镜。该系统是 Zeiss LSM 510 Meta 与 Axiovert 200 MOT 相结合，将取代已有 10 年历史的 LSM 410 系统，拥有来自 29 个部门的 232 名注册用户。所请求的系统将由我们大学最古老的成像核心设施神经科学成像中心 (NIC) 安置和管理。 NIC 聘用了一名拥有 8 年 LSM 410 共焦显微镜操作经验的全职技术人员。描述了 8 个主要用户和 9 个次要用户的项目。这些项目将受益于 LSM 510 Meta 改进的光学和检测系统，从而改善荧光染料发射的捕获、光谱分离、降低光毒性以及进行荧光共振能量转移 (FRET) 测量的能力。主要用户的项目包括：大鼠和小鼠海马切片或培养物中 Ca 2+ 通道的运输（Dr. Herlitze）、肌球蛋白 II 在人类癌细胞运动过程中的作用和机械功能（Dr. Egelhoff）、开发新的小鼠品系作为脑血清素能神经元研究的遗传工具（Deneris 博士）、5-HT 受体靶向和运输的调节（Roth 博士）、作用黑腹果蝇内吞周期中动力相互作用蛋白的作用（Robinson 博士）、内吞作用在 EGF 受体运输中的作用（Carlin 博士）、出生后小脑分化的调节（Siegel 博士）和跨神经胶质疤痕的轴突再生（Silver 博士） ）。