Imaging Research Core

影像研究核心

• 批准号: 10611854
• 负责人: Gregg W Roman
• 金额: $ 22.18万
• 依托单位: UNIVERSITY OF MISSISSIPPI
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-05-15 至 2025-03-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10611854
• 关键词: Address; Awareness; Biological; Biological Assay; Biotinylation; Carbohydrates; Cell physiology; Cells; Cellular Structures; Cellular biology; Centers of Research Excellence; Chemicals; Chemistry; Complex; Computer Assisted; Computer Workstations; Computer software; Computers and Advanced Instrumentation; Confocal Microscopy; Consult; Data; Development; Dimensions; Disease; Ensure; Extracellular Structure; Fiji; Fluorescence Resonance Energy Transfer; Goals; Health; Human; Image; Image Analysis; Imaging Techniques; Individual; Instruction; Label; Laser Scanning Confocal Microscopy; Lectin; Location; Maintenance; Maps; Measures; Methods; Microscope; Microscopic; Microscopy; Mississippi; Modernization; Molecular; Motor; Organism; Phase-Contrast Microscopy; Physiological; Polysaccharides; Preparation; Property; Protocols documentation; Reagent; Research; Research Personnel; Role; Sampling; Schedule; Specific qualifier value; Specificity; Subcellular structure; System; Techniques; Tissues; Training; Training Programs; Universities; automated analysis; imager; imaging platform; imaging system; in vivo; insight; instrument; instrumentation; molecular dynamics; novel strategies; operation; particle; physical property; preservation; programs; protein protein interaction; protein transport; response; superresolution imaging; tool

There is an unequivocal need to develop and employ advanced techniques in microscopy toward understanding the cellular functions of glycans. Glycans participate in many cellular properties, including cell structure, cell-cell recognition, protein trafficking, and protein-protein interaction. Hence, changes in glycan abundance or composition can have considerable effects on cellular function, and as a consequence human health. Advanced imaging techniques are becoming a vital part of an integrative approach to understanding the roles of glycans in health and disease. These techniques provide insights into molecular composition, while preserving the spatial context of the chemical, structural, and physiological responses to changes in glycans. Recent advances in identify lectin specificities and the availability of a broad range of fluorescently labeled and biotinylated lectins has rapidly advanced our ability to identify changes in the location and abundance of specific glycans in cells and tissues. Moreover, several powerful approaches in confocal microscopy, including FRET, FRAP, and FLIP, permit highly sensitive measures of molecular dynamics within living cells. These measures will help researchers understand the fundamental effect glycans have on cellular function. The central goal of this proposal is to bring the ability to employ advanced imaging techniques to the GlyCORE investigators in their diverse research programs and to help develop new approaches in the cell biology of glycans. These goals will be accomplished in three aims: 1) establish an imaging core that contains three different imaging platforms, including a powerful new confocal microscope; 2) support the GlyCORE investigators in their individual projects by working to develop new imaging applications; and 3) develop an extensive training program to develop expertise and awareness of new methods in imaging and image analysis. This proposal will transform research in the glycosciences at the University of Mississippi by providing access to advanced instrumentation and expertise and training in the use of these imaging systems.

在显微镜中开发和采用高级技术的需求明确 了解聚糖的细胞功能。 Glycans参与许多细胞特性，包括细胞 结构，细胞细胞识别，蛋白质运输和蛋白质 - 蛋白质相互作用。因此，聚糖的变化 丰度或成分可能会对细胞功能产生重大影响，因此人类 健康。先进的成像技术正在成为理解的综合方法的重要组成部分 聚糖在健康和疾病中的作用。这些技术提供了有关分子组成的见解， 同时保留了化学，结构和生理反应的空间环境对变化的变化 聚糖。识别凝集素特异性的最新进展以及广泛的荧光范围的可用性 标记和生物素化的凝集素已迅速提高了我们确定位置变化的能力， 细胞和组织中的特定聚糖丰度。而且，共焦的几种有力的方法 显微镜，包括FRET，FRAP和FLIP，允许高度敏感的分子动力学测量 活细胞。这些措施将帮助研究人员了解聚糖对细胞的基本影响 功能。该提案的核心目标是使采用高级成像技术的能力 聚糖研究人员在各种研究计划中，并帮助开发细胞中的新方法 聚糖的生物学。这些目标将以三个目的实现：1）建立一个包含的成像核心 三个不同的成像平台，包括强大的新共聚焦显微镜； 2）支持聚糖 通过努力开发新成像应用程序，调查人员在其各个项目中； 3）发展 广泛的培训计划，以发展成像和图像中新方法的专业知识和意识 分析。该提议将改变密西西比大学糖基镜的研究 在使用这些成像系统的使用中，可访问先进的仪器，专业知识和培训。