CALCIUM-MEDIATED EXOCYTOSIS OF NEUROTRANSMITTER DURING SYNAPTIC TRANSMISSION

突触传递过程中钙介导的神经递质胞吐作用

• 批准号: 7722315
• 负责人: WILLIAM M ROBERTS
• 金额: $ 0.32万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-05-01 至 2009-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7722315
• 关键词: Address; Calcium; Cell membrane; Chemical Synapse; Chromosome Pairing; Class; Computer Retrieval of Information on Scientific Projects Database; Cytoplasm; Dependence; Dimensions; Electrons; Exocytosis; Funding; Goals; Grant; Hair Cells; Institution; Lead; Location; Measures; Mediating; Modeling; Neurotransmitters; Numbers; Organelles; Physiological; Research; Research Personnel; Resources; Source; Synapses; Synaptic Transmission; Synaptic Vesicles; Testing; United States National Institutes of Health; Vesicle; Work; body map; mathematical model; presynaptic; synaptic function

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The long-term goal of these studies is to understand the presynaptic mechanisms of transmitter release at fast chemical synapses. Our specific objective is to reconstruct in three dimensions the ultrastructure of the hair cell afferent synapse in order to test several aspects of our working model of this synapse's function. The hair cell afferent synapse is a ribbon-class synapse, characterized by an electron dense organelle of unknown function, the synaptic body, which appears to hover in the cytoplasm a vesicle's breadth above the active zone. Clear-core vesicles surround the synaptic body, but are also found in the cytoplasm, and associated with the plasma membrane at the active zone. What is the function of the synaptic body, and what is the functional significance of these various pools of vesicles? We will address these issues by determining the precise geometry of the active zone and synaptic body, and map the number and location of synaptic vesicles. By testing the activity-dependence of these measures, we will be able to integrate ultrastructure with ongoing physiological and mathematical modeling studies to build a more detailed understanding of the steps that lead to transmitter exocytosis.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 这些研究的长期目标是了解快速化学突触中发射机释放的突触前机制。我们的具体目标是在三个维度上重建毛细胞传入突触的超微结构，以测试我们突触功能的工作模型的几个方面。毛细胞传入的突触是丝带级的突触，其特征是一个未知功能的电子密集的细胞器，突触体，似乎在活性区上的细胞质A囊泡宽度上悬停。明确囊泡围绕着突触体，但也存在于细胞质中，并与活性区的质膜有关。突触体的功能是什么，这些各种囊泡的功能意义是什么？我们将通过确定活性区和突触体的精确几何形状，并绘制突触囊泡的数量和位置来解决这些问题。通过测试这些措施的活动依赖性，我们将能够将超微结构与持续的生理和数学建模研究整合在一起，以对导致发射机胞吐作用的步骤建立更详细的理解。