Core--Molecular recognition

核心--分子识别

• 批准号: 6395767
• 负责人: RAYMOND L MERNAUGH
• 金额: --
• 依托单位: VANDERBILT UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-09-01 至 2000-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6395767
• 关键词: analytical chemistry; biomedical equipment resource; biomedical facility; macromolecule; monoclonal antibody; neoplasm /cancer; oncology; proteins

The Molecular Recognition Shared Resource (MRSR) is a newly established core facility. The goal of the MRSR is to provide investigators with reagents, equipment, and expertise for the production, detection and characterization of biomolecules. The MRSR is composed of three individual Resources , the Antibody Resource, the Protein Expression Resource and the BIAcore Resource. Each of these resources provides unique, cost- effective services and expertise for Vanderbilt Cancer Center (VCC) and University Investigators. The goals and the objectives of three resources are described below. The goal of the Antibody Resource is to provide the expertise, reagents and equipment for the production, detection, characterization, purification and labeling of traditional polyclonal, monoclonal and phage-displayed or soluble ScFv recombinant antibodies. Assays incorporating antibodies can also be developed to detect and/or quantitate proteins, carbohydrates or nucleic acids which occur individually or as components of a biological milieu. The objective of the Antibody Resource is to provide antibodies and antibody-based assays to VCC and University Investigators on a timely and cost-effective basis. The goal of the Protein Expression Resource is to provide VCC and University Investigators with reagents and equipment for large scale protein expression using the baculovirus expression system. Additionally, Investigators are provided with equipment necessary to harvest large amounts of cellular material. The goal and objective of the BIAcore Resource is to provide VCC and University Investigators with access to the BIAcore 2000, an instrument from BIAcore, AB., that performs real-time biomolecular interaction analysis using surface plasmon resonance technology.

分子识别共享资源（MRSR）是新成立的核心设施。 MRSR的目的是为研究人员提供生物分子生产，检测和表征的试剂，设备和专业知识。 MRSR由三个个人资源，抗体资源，蛋白质表达资源和Biacore资源组成。 这些资源中的每一个都为范德比尔特癌症中心（VCC）和大学调查人员提供了独特的，具有成本效益的服务和专业知识。 下面描述了三个资源的目标和目标。抗体资源的目的是为传统多克隆，单克隆和噬菌体播放或可溶性SCFV重组抗体的生产，检测，表征，纯化和标记提供专业知识，试剂和设备。 还可以开发掺入抗体的测定方法来检测和/或定量单独或作为生物环境成分的蛋白质，碳水化合物或核酸。 抗体资源的目的是向VCC和大学研究人员及时且具有成本效益的基础提供抗体和基于抗体的测定。蛋白质表达资源的目的是使用杆状病毒表达系统为VCC和大学研究人员提供大规模蛋白质表达的试剂和设备。此外，还为研究人员提供了收集大量细胞材料所需的设备。 BIACORE资源的目标和目标是为VCC和大学研究人员提供使用Biacore 2000（来自AB的Biacore的仪器）的Biacore 2000，该仪器使用表面等离子体元共振技术进行实时的生物分子交互分析。