Molecular Biology and Analytical Core

分子生物学和分析核心

基本信息

批准号：
8376988
负责人：
WILLIAM H BEIERWALTES
金额：
$ 24.21万
依托单位：
HENRY FORD HEALTH SYSTEM
依托单位国家：
美国
项目类别：
财政年份：
2012
资助国家：
美国
起止时间：
2012-04-01 至 2014-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8376988
关键词：
1-Phosphatidylinositol 3-Kinase 2-Mercaptoethanol Accidents Acetates Acids Adenosine Adenovirus Vector Adenoviruses Adenylate Cyclase Affect Analytical Chemistry Angiotensin I Angiotensinogen Antibodies Back Bathing Binding Biological Biological Assay Biomedical Technology CMV promoter Calcium California Calmodulin Camping Cations Cells Charge Cholates Cities Clinical Chemistry Cloning Color Complementary DNA Computer software Computers Consensus Sequence Crotalus Cultured Cells Cyan Fluorescent Protein Cyclic GMP DNA Data Detection Detergents Dose Edetic Acid Egtazic Acid Electrolytes Ensure Enzyme-Linked Immunosorbent Assay Enzymes Equipment Escherichia coli Faculty Firefly Luciferases Fluorescence Resonance Energy Transfer Freedom Freezing Frozen Sections Furuncles Generations Genes Genomics Genotype Glass Glyceraldehyde 3-Phosphate Griess reagent Guanosine Harvest Hospitals Hour Immunoassay Immunosorbents Incubated Individual Instruction Ion Exchange Ion-Exchange Chromatography Procedure Isoenzymes Juxtaglomerular Cell Kidney Label Laboratories Lasers Learning Letters Leupeptins Light Limb structure Link Liquid substance Location Luciferases Macula densa Mammalian Cell Manuals Manufacturer Name Measurement Measures Membrane Messenger RNA Methods Microscope Modeling Modification Molecular Molecular Analysis Molecular Biology Mus Mutation Nitrate Reductases Nitrates Nitric Oxide Synthase Nitric Oxide Synthase Type I Nitrites Nucleosides One-Step dentin bonding system PDE2 phosphodiesterase Paint Particulate Pepstatins Peptides Peroxidases Phosphatidylinositols Phospho-Specific Antibodies Phosphoric Acids Phosphorylation Phosphoserine Phosphothreonine Phosphotransferases Physiological Plasma Plasmids Preparation Procedures Productivity Proteins Proteomics Proto-Oncogene Proteins c-akt Quality Control RNA Radioimmunoassay Reaction Reader Reading Reagent Recovery Relative (related person)Renal Hypertension Renin Reporter Reporting Research Research Personnel Residual state Resource Sharing Reverse Transcriptase Polymerase Chain Reaction Ribonucleases Role Rolipram Running Sampling Sepharose Serine Serum Services Shapes Sheep Shipping Ships Shuttle Vectors Signal Transduction Site Small Interfering RNA Snake Venoms Sodium Sodium Chloride Solutions Specimen Staining method Stains Staurosporine Streptavidin Sucrose Sulfanilamide Suspension substance Suspensions Tail Techniques Technology Teflon Testing Thick Threonine Time Tissues Toxin Tracer Transfection Transplantation Universities Virginia Water Weight Width Yang adenylyl cyclase 6 animal facility antibody conjugate base blood pressure regulation chromophore cilostamide cost data reduction design detector enzyme activity ethane sulfonate experience gene cloning human NOS3 protein indexing inhibitor/antagonist interest isopentane kidney cortex knockout animal laboratory facility laser capture microdissection leupeptin luciferin medical schools member nitrate reductase novel oxidation pepstatin phosphodiesterase IV phosphodiesterase V phosphoric diester hydrolase prevent programs promoter receptor research study restriction enzyme sensor sephadex vardenafil vector

项目摘要

The purpose of the Molecular Biology and Analytical Core (Core B) is to centralize molecular analysis, cloning, laser capture microdissection, radioimmunoassays (RIA), enzyme activity assays, and analytical chemistry. Analytical chemistry, radioimmunoassay, enzyme immunosorbent assays, real time RT-PCR and cloning of siRNAs into shuttle vectors are of fundamental importance for state-of-the-art hypertension and renal research. Core B will perform a number of labor-intensive and time-consuming analyses that will relieve investigators from having to conduct these assays themselves and thereby allow them more time to pursue their scientific efforts. The molecular biology aspect of the core will aid investigators by cloning, performing laser capture microdissection, real time RT-PCR and genotyping knockout animals. The analytical aspect of the core will use RIA to measure cAMP and cGMP, and will also determine Ang I as an index of renin activity and/or concentration. Enzyme activity assays will also be performed for phosphodiesterase activity, phosphatidylinositol 3 kinase (PIS kinase) activity, and Akt activity. Analytical chemistry will be performed to determine calcium and sodium concentrations. Centralizing of analytical and molecular studies in Core B will ensure cost efficiency in terms of labor and laboratory facilities/equipment by eliminating duplication of effort by individual investigators. It will also help integrate projects by providing consistent and reliable assays which promote both quality and productivity for participating projects.

分子生物学和分析核心（核心B）的目的是集中分子分析，克隆，激光捕获微分解，放射免疫测定（RIA），酶活性测定和分析化学。分析化学，放射免疫测定，酶免疫吸附测定，实时RT-PCR和将siRNA克隆到航天飞机载体中对于最先进的高血压和肾脏研究。核心B将执行许多劳动密集型和耗时的分析减轻调查人员必须自己进行这些测定，从而使他们有更多时间追求他们的科学努力。核心的分子生物学方面将通过克隆来帮助研究者执行激光捕获显微解剖，实时RT-PCR和基因分型基因敲除动物。这核心的分析方面将使用RIA测量CAMP和CGMP，还将确定ANG I作为一个肾素活性和/或浓度的指数。酶活性测定也将进行磷酸二酯酶活性，磷脂酰肌醇3激酶（PIS激酶）活性和AKT活性。分析将进行化学以确定钙和钠浓度。分析和核心B中的分子研究将确保劳动力和实验室设施/设备的成本效率消除单个研究人员的努力重复。它还将通过提供来帮助整合项目一致且可靠的测定法，促进参与项目的质量和生产力。