MOLECULAR RECOGNITION & SCREENING SHARED RESOURCE

分子识别

基本信息

批准号：
7679669
负责人：
RAYMOND L MERNAUGH
金额：
$ 36.99万
依托单位：
VANDERBILT UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
资助国家：
美国
起止时间：
至
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/7679669
关键词：
Amines Animals Antibodies Antibody Formation Antigens Bacteriophages Baculovirus Expression System Baculoviruses Biochemical Biological Biological Assay Biology Biotin Breast Budgets Cancer Center Support Grant Carbohydrates Cells Chemicals Coupling Culture Media Cultured Cells Daily Data Analyses Detection Development Enzymes Equipment Escherichia coli Fluorescent Probes Freezing Funding Generations Genetic Glass Growth Hemorrhage Human Human Resources Hybridomas Immunization Immunoassay Immunohistochemistry Immunoprecipitation In Vitro Incubators Insecta Institutes Institution Instruction Kinetics Laboratories Libraries Ligands Mass Spectrum Analysis Molecular Toxicology Monoclonal Antibodies Mus Nucleic Acids Operative Surgical Procedures Performance Phage Display Preclinical Drug Evaluation Preparation Procedures Production Proteins Protocols documentation Range Rattus Reagent Recombinant Antibody Recombinants Recruitment Activity Reporter Reproduction spores Research Personnel Resource Sharing Rodent Role Sampling Screening procedure Serum Services Source Spleen Sterility Streptavidin Sulfhydryl Compounds Surface Plasmon Resonance Testing The Vanderbilt-Ingram Cancer Center at the Vanderbilt University Thermodynamics Time Training Western Blotting antibody conjugate base cost day experience flasks high throughput screening instrument instrumentation member molecular recognition monoclonal antibody production protein expression scale up sensor small molecule libraries tissue culture vector

项目摘要

The Molecular Recognition and Screening Shared Resource (MRSSR) provides VICC investigators with reagents, equipment, and expertise for the production, detection, and characterization of biomolecules. A major role for the MRSSR is to generate monoclonal and phage-displayed single-chain (ScFv) antibodies and to assist investigators in their use in a range of applications. Shared Resource staff also assist in the expression of protein antigens via insect cell expression, scale-up of monoclonal antibody production by growth of hybridomas, and scale-up of ScFvs by expression in E. coli. The insect cell culture facility is also used for high capacity protein expression for biophysical studies and drug screening. Beginning in early 2004, MRSSR staff will assist investigators in the performance of high throughput screens of a large chemical library for activities in vitro or in cell-based assays. Dr. Ray Mernaugh oversees the daily operations of the MRSSR. He has extensive experience in monoclonal and phage-displayed antibody generation as well as protein expression. He meets with users, trains MRSSR personnel, and oversees and coordinates the MSSR's budget, equipment, and use of facilities and services. He is assisted by a highly-trained and experienced staff who are able to help VICC investigators.

分子识别和筛选共享资源（MRSSR）为VICC研究人员提供了生物分子生产，检测和表征的试剂，设备和专业知识。 MRSSR的主要作用是产生单克隆和噬菌体播种的单链（SCFV）抗体，并协助研究人员在一系列应用中使用。共享的资源人员还通过昆虫细胞表达，通过杂交瘤的生长进行昆虫细胞的表达，单克隆抗体产生的扩大以及通过大肠杆菌中表达来扩大SCFV的表达。昆虫细胞培养设施还用于高容量的蛋白质表达，用于生物物理研究和药物筛查。从早期开始 2004年，MRSSR工作人员将协助研究人员进行大型化学库的高吞吐量屏幕，以在体外或基于细胞的测定中进行活动。 Ray Mernaugh博士负责MRSSR的日常操作。他在单克隆和噬菌体播种抗体产生以及蛋白质表达方面具有丰富的经验。他会见用户，火车 MRSSR人员，并监督和协调MSSR的预算，设备和设施和服务的使用。他得到了一位经验丰富且经验丰富的员工的协助，他们能够帮助VICC调查人员。