Core--Molecular Recognition

核心--分子识别

• 批准号: 6725960
• 负责人: RAYMOND L MERNAUGH
• 金额: $ 11.47万
• 依托单位: VANDERBILT UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-04-01 至 2009-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6725960
• 关键词: biomedical equipment resource; biomedical facility; biotechnology; environmental toxicology; health science research support; high throughput technology; hybridomas; immunologic substance development /preparation; intermolecular interaction; molecular shape; monoclonal antibody; nucleic acid quantitation /detection; peptide chemical synthesis; protein quantitation /detection; reagent /indicator

Description (provided by applicant): The Molecular Recognition Core Facility consists of two parts. One part contains equipment and space that was a part of the previous "Molecular Biology Core Facility " and continues to be extensively utilized by Center investigators in their projects. The overall goal is to provide needed equipment and services in a cost-effective manner. The other component is a joint facility designed to provide new, essential high-throughput services related to the general area of molecular recognition to both Center and University investigators. The goal of the Molecular Recognition Core Facility is to provide investigators with reagents and equipment for the production, detection, and characterization of biomolecules. The facility is composed of three "Resources," the Antibody Resource, the Protein Expression Resource, and the BIAcore Resource. Each Resource provides unique, cost-effective services and expertise for Center and other University investigators. The goal of the Antibody Resource is to provide the expertise, reagents, and equipment for the production, detection, characterization, purification, and labeling of monoclonal and recombinant phage-displayed or soluble ScFv antibodies for research, diagnostic, or therapeutic use. Assays incorporating antibodies can also be developed to detect and/or quantify proteins, carbohydrates, or nucleic acids that occur as components of a biological or chemical milieu. The objective of the Antibody Resource is to provide hybridoma monoclonal and phage-displayed recombinant antibodies and antibody-based assays to Center investigators and other individuals throughout the University on a timely and cost-effective basis. The goal of the Antibody Resource is to provide the expertise, reagents, and equipment for the production, detection, characterization, purification, and labeling of monoclonal and recombinant phage-displayed or soluble ScFv antibodies for research, diagnostic, or therapeutic use. Assays incorporating antibodies can also be developed to detect and/or quantify proteins, carbohydrates, or nucleic acids that occur as components of a biological or chemical milieu. The objective of the Antibody Resource is to provide hybridoma monoclonal and phage-displayed recombinant antibodies and antibody-based assays to Center investigators and other individuals throughout the University on a timely and cost-effective basis. The goal of the Protein Expression Resource is to provide Center and other University investigators with reagents and equipment for large-scale protein production using the baculovirus expression system. Additionally, the Protein Expression Resource also provides investigators with equipment necessary to harvest large amounts of cellular material. The goal and the objective of the BIAcore Resource is to provide Center and other University investigators with access to the BIAcore 2000, an instrument from BIAcore Inc., that performs real-time biomolecular interaction analysis.

描述（由申请人提供）：分子识别核心设施由两个部分组成。一部分包含设备和空间，这是以前“分子生物学核心设施”的一部分，并继续被中心研究人员在其项目中广泛使用。总体目标是以具有成本效益的方式提供所需的设备和服务。另一个组件是一个联合设施，旨在提供与中心和大学研究人员的分子识别一般领域有关的新的，基本的高通量服务。 分子识别核心设施的目的是为研究人员提供试剂和 生物分子生产，检测和表征的设备。该设施由三个“资源”，抗体资源，蛋白质表达资源和Biacore资源组成。每种资源为中心和其他大学调查人员提供独特的，具有成本效益的服务和专业知识。抗体资源的目的是为该抗体提供专业知识，试剂和设备 单克隆和重组的生产，检测，表征，纯化和标记 用于研究，诊断或治疗用途的噬菌体播种或可溶性SCFV抗体。测定 还可以开发掺入抗体来检测和/或定量作为生物或化学环境成分的蛋白质，碳水化合物或核酸。抗体资源的目的是为及时且具有成本效益的整个大学提供单克隆和噬菌体播种的重组抗体和基于抗体的分析的杂交瘤。抗体资源的目的是为生产，检测，表征，纯化和标记提供专业知识，试剂和设备，用于研究，诊断或治疗用途的单克隆和重组噬菌体播种或可溶性SCFV抗体。还可以开发掺入抗体的测定方法来检测和/或量化蛋白质，碳水化合物或 作为生物或化学环境的成分出现的核酸。抗体资源的目的是为及时且具有成本效益的整个大学提供单克隆和噬菌体播种的重组抗体和基于抗体的分析的杂交瘤。蛋白质表达资源的目的是为中心和其他大学研究人员提供使用杆状病毒表达系统生产大规模蛋白质的试剂和设备。此外，蛋白质表达资源还为研究人员提供了收集大量细胞材料所需的设备。 BIACORE资源的目标和目的是为中心和其他大学研究人员提供Biacore 2000（来自Biacore Inc.的仪器）的访问，该工具可执行实时的生物分子交互分析。