QP21 Q-PIX COLONY PICKER

QP21 Q-PIX 菌落挑选器

• 批准号: 6291658
• 负责人: RAYMOND L MERNAUGH
• 金额: $ 11.37万
• 依托单位: VANDERBILT UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-04-01 至 2002-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6291658
• 关键词: biomedical equipment; biomedical equipment purchase; high throughput technology; microorganism culture

This application requests funds for the purchase of a Genetic QP-21 Qpix robotic colony picker equipped with a re-arraying and replication package. The advantages of this type of instrument are currently unavailable at Vanderbilt University Medical Center. This bench top instrument is capable of aseptically picking greater than 3,000 bacterial colonies or viral plaques from agar surfaces and transferring them to 96 or 384 well microliter plates. The instrument is capable of re-arraying positive clones from fifteen 96 or 384 well plates into 96 or 384 well plates. This instrument will be incorporated into the Molecular Recognition Unit (MRU). The MRU is an established university shared resource that is supported by NIH Cancer and Molecular Toxicology Center Grants and institutional funds. The MRU's primary function is the production and characterization of monoclonal antibodies and phage- displayed and soluble recombinant antibodies for research, diagnostic or therapeutic use. Members of the MRU, the departments of Microbiology and Immunology, Medicine, the Cancer Center, the Center in Molecular Toxicology, and the newly establishes, university funded DNA Micro array Core will utilize this instrument. Projects supported by this instrument will address studies involving signal transduction, vascular biology, neuroscience, virology, oncology and toxicology. Major users will benefit from this instrument in studies to determine the functional pharmacogenomics of human P450 enzymes; or will utilize the instrument through the MRU to develop antibodies to identify radiation- induced cell surface antigens, to identify viral antigens and cell receptors and to detect damaged DNA (DNA adducts). Minor users will utilize the instrument to analyze c-DNA libraries, re-array bacterial clones for the production of DNA micro arrays or utilize the MRU to obtain recombinant antibodies to novel antigens on an occasional basis.

该申请要求购买遗传QP-21 QPIX机器人殖民地采摘器，配备了重新出入和复制套件。范德比尔特大学医学中心目前无法使用这种类型的乐器。这种台式仪器能够从琼脂表面挑选3,000多个细菌菌落或病毒斑块，并将其转移到96或384个井井板上。该仪器能够从15个96或384井板中重新列出正板，以96或384孔板。该仪器将纳入分子识别单元（MRU）。 MRU是一项已建立的大学共享资源，由NIH癌症和分子毒理学中心赠款和机构资金支持。 MRU的主要功能是用于研究，诊断或治疗用途的单克隆抗体和噬菌体的产生和表征。 MRU的成员，微生物学和免疫学部门，医学，癌症中心，分子毒理学中心以及新建立的，大学资助的DNA微阵列核心将利用该工具。该工具支持的项目将解决涉及信号转导，血管生物学，神经科学，病毒学，肿瘤学和毒理学的研究。主要用户将从研究中受益，以确定人类P450酶的功能性药物基因组学；或将通过MRU利用该仪器开发抗体来鉴定辐射诱导的细胞表面抗原，以鉴定病毒抗原和细胞受体并检测受损的DNA（DNA加合物）。次要用户将利用该仪器分析C-DNA文库，重新列出细菌克隆来生产DNA微阵列，或者使用MRU偶尔获得对新型抗原的重组抗体。