LabTM IC100 High Throughput Microscopy System

LabTM IC100 高通量显微镜系统

• 批准号: 6879374
• 负责人: LESTER KOBZIK
• 金额: $ 24.1万
• 依托单位: HARVARD SCHOOL OF PUBLIC HEALTH
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-06-01 至 2006-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6879374
• 关键词: biomedical equipment purchase; flow cytometry; high throughput technology; molecular /cellular imaging; biomedical equipment purchase; flow cytometry; high throughput technology; molecular /cellular imaging

DESCRIPTION (provided by applicant): Equipment Requested: This proposal seeks support for acquisition of a Cell Lab TM IC100 High Throughput Microscopy System made by the Q3DM Beckman Coulter. The system will allow high-throughput 'scanning cytometry' of adherent cells and tissue sections by a large number of NIH-supported researchers. Rationale: Scanning cytometry brings the power of quantitative fluorescence measurements of thousands of cells to bear on analysis of adherent cells in culture and tissue samples. The user group will perform analyses of cell cycle, antigen expression, apoptosis, phagocytosis and cytoskeletal and tissue morphometry using fluorescent probes and the automated digital image capturing abilities of this scanning cytometry system. The scanning cytometry approach will offer investigators the ability to perform high-throughput analyses without harsh treatments and artifacts introduced in preparing adherent cells for suspensions and flow cytometry. New methods for automated analysis of environmental particles will be explored. Implementation: The instrumentation will be situated within a well-established bioimaging core facility, supported by an NIEHS Center grant. The core is managed by two scientists (the PI and co-PI for this application) with experience in core facility management and in developing new methodologies for user benefit. Two senior technologists with experience in flow cytometry and confocal microscopy will be trained on the system and will provide training, supervision and support to the user group lab members. Support includes newly renovated space for the core facility (institutional), coverage of service contracts for the system (institutional), and core facility support (NIEHS Center grant). An internal advisory committee will supervise use and assure access to the system. Summary: This instrumentation will significantly improve the research capacity of a large number of NIH-supported investigators via an institutionally supported, well-established core facility.

描述（由申请人提供）：要求的设备：该建议寻求支持以获取由Q3DM Beckman Coulter制造的单元实验室TM IC100高吞吐量显微镜系统。该系统将允许大量支持NIH的研究人员对粘附细胞和组织切片的高通量“扫描细胞仪”。理由：扫描细胞仪带来了数千个细胞的定量荧光测量的功能，以分析培养和组织样品中粘附细胞的分析。用户组将使用荧光探针以及该扫描细胞计量学系统的自动数字图像捕获能力，对细胞周期，抗原表达，凋亡，吞噬作用以及细胞骨架和组织形态学分析。扫描细胞仪方法将使研究人员能够进行高通量分析的能力，而无需在准备悬浮液和流式细胞仪的粘附细胞中引入的严厉处理和伪影。将探索用于自动分析环境颗粒的新方法。实施：该仪器将位于由NIEHS中心赠款的支持下，建立在良好的生物成像核心设施中。核心由两位科学家（本应用程序的PI和Co-Pi）管理，并具有在核心设施管理中的经验以及为用户利益开发新方法。两名具有流式细胞仪和共聚焦显微镜经验的高级技术人员将在系统上进行培训，并将为用户组实验室成员提供培训，监督和支持。支持包括核心设施新近装修的空间（机构），系统的服务合同（机构）和核心设施支持（NIEHS中心拨款）。内部咨询委员会将监督使用并确保对系统的访问。摘要：该仪器将通过机构支持的，公认的核心设施大大提高大量NIH支持的研究人员的研究能力。