CENTER FOR BIOMEDICAL MASS SPECTROMETRY

生物医学质谱中心

• 批准号: 2902224
• 负责人: CHARLES R IDEN
• 金额: $ 23.82万
• 依托单位: STATE UNIVERSITY NEW YORK STONY BROOK
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-09-01 至 2004-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2902224
• 关键词: biomedical equipment purchase; biomedical equipment resource; electrospray ionization mass spectrometry; gas chromatography mass spectrometry; health science research; mass spectrometry; neoplasm /cancer; oncoproteins

Tremendous technical advances have led to the discovery of new gene products, and the natural progression to the investigation of protein function and mechanism in carcinogenesis will unquestionably follow and will require sensitive new analytical tools. Mass spectrometry will have a critical role in these determinations due to the unparalleled sensitivity of new ionization techniques and the potential for sequencing biopolymers. This proposal requests funds to establish a Center for Biomedical Mass Spectrometry (CBMS) in the School of Medicine that will greatly facilitate the research programs of ten eminent investigators working in chemical carcinogenesis, DNA repair, mitochondrial/nuclear proteins, and cell signaling mechanisms. Newly acquired state-of-the-art instrumentation is available including systems for electrospray ionization LC/MS and LC/MS/MS, and matrix assisted laser desorption. Trained mass spectrometrists will work directly with senior investigators and their group members. Projects will be undertaken to perform non-standard analyses that require considerable development of experimental parameters and techniques. Incorporation of new technologies at the cutting edge of mass spectrometry will be essential for these investigators and will be undertaken by senior personnel. An administrative structure is described that will ensure a fully functioning Center with optimal communication and with access for all NCI-supported investigators. In addition, the Center will provide leadership in the education of basic biomedical scientists through a series of seminars on biomedical applications and new technologies and by providing instruction in mass spectrometry techniques to students in graduate and undergraduate courses.

巨大的技术进步导致发现了新的基因产物，并且在癌变中研究蛋白质功能和机制的自然发展将无疑是遵循的，并且需要敏感的新分析工具。 由于新电离技术的无与伦比的灵敏度和测序生物聚合物的潜力，质谱法将在这些确定中起关键作用。 该提案要求资金在医学院建立生物医学质谱中心（CBM），这将极大地促进了从事化学癌变，DNA修复，线粒体/核蛋白质和细胞信号机制的十位著名研究人员的研究计划。可获得新获得的最新仪器，包括用于电喷雾的LC/MS和LC/MS/MS的系统，以及矩阵辅助激光解吸。 训练有素的质谱师将直接与高级调查员及其小组成员合作。 将进行项目，以进行非标准的分析，这些分析需要大量发展实验参数和技术。 在质谱法的最前沿纳入新技术对于这些研究人员至关重要，并且将由高级人员进行。 描述了一种行政结构，该结构将确保具有最佳通信的功能齐全的中心，并为所有NCI支持的研究人员提供访问。 此外，该中心将通过一系列有关生物医学应用和新技术的研讨会以及通过向研究生和本科课程的学生提供质谱技术的教学，通过一系列有关生物医学应用和新技术的研讨会来教育基础生物医学科学家的领导。