Acquisition of a Thermo Scientific Orbitrap XL Hybrid FT Mass Spectrometer

购买 Thermo Scientific Orbitrap XL 混合 FT 质谱仪

基本信息

批准号：
7498266
负责人：
CHARLES R IDEN
金额：
$ 85.09万
依托单位：
STATE UNIVERSITY NEW YORK STONY BROOK
依托单位国家：
美国
项目类别：
财政年份：
2008
资助国家：
美国
起止时间：
2008-07-01 至 2010-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7498266
关键词：
Advisory Committees Atmospheric Pressure Biochemical Markers Biomedical Research Biopolymers Chemicals Collection Coupling Daily Data Diabetes Mellitus Disease Dissociation Electron Transport Ensure Funding Heart Diseases High Pressure Liquid Chromatography Human Resources Hybrids Information Systems Injection of therapeutic agent Ions Knowledge Laboratories Link Lipids Liquid substance Malignant Neoplasms Measurement Methods Oligonucleotides Oligosaccharides Operative Surgical Procedures Peptides Pharmaceutical Preparations Post-Translational Protein Processing Productivity Proteins Proteomics Public Health Request for Proposals Research Research Personnel Resolution Schedule Scientist Source System Technical Expertise Techniques Training Universities data acquisition human disease innovation instrument ionization liquid chromatography mass spectrometry mass spectrometer medical schools nano small molecule

项目摘要

DESCRIPTION (provided by applicant): This proposal requests funds for the purchase of a Thermo Scientific LTQ Orbitrap Hybrid FT Mass Spectrometer and Data System. The instrument is linked directly to a Dionex Nano HPLC system affording LC/MS and (MS)n capability and will be located in the Proteomics Center in the Stony Brook School of Medicine. This system will be provide high resolution, accurate mass measurement and electron transfer dissociation, two new mass spectrometer techniques that will significantly promote the research objectives and laboratory productivity of thirteen distinguished scientists from Stony Brook University (SBU) who comprise the major users group. These methods are presently unavailable at SBU and inaccessible or difficult to obtain from other organizations. LC/MS/MS is unsurpassed for the characterization of molecules with a vital biomedical significance, including lipids, biopolymers such as peptides, oligonucleotides, and oligosaccharides and smaller compounds which are non-volatile, labile, or thermally unstable. Techniques for coupling a liquid chromatograph to a mass spectrometer through electrospray or atmospheric pressure chemical ionization sources now permit the routine analysis of these substances with ease and rapidity. Collection of accurate mass data will facilitate protein and small molecule characterization, while the mass analysis of ETD-generated fragment ions will make possible more complete characterization of protein post-translational modifications. New information dependent data acquisition routines permit complete analysis of multiple compounds during a single injection. Quantitative methods employing sensitive LC/MS/MS and iTRAQ techniques are essential for several investigators. The instrument will be operated and maintained by trained personnel at the Proteomics Center, and the Director will schedule daily operation and provide technical expertise for the users. An advisory committee will ensure access to the major users and other PHS-supported investigators and will be responsible for the long term operation of the instrument. PUBLIC HEALTH RELEVANCE Innovative biomedical research requires frequent access to the newest analytical techniques to advance knowledge of human diseases and to provide new treatments for these illnesses. The Thermo Scientific LTQ Orbitrap Hybrid FT Mass Spectrometer and Data System will permit low-level analysis of many biomolecules critical to the research efforts of NIH-supported investigators at Stony Brook. They will use the new, analytical techniques (accurate mass measurement and electron transfer dissociation) offered by this instrument to explore mechanisms leading to diabetes, cancer, and heart disease and help define new biochemical markers and medications for these disease states.

描述（由申请人提供）：该提案请求资金用于购买 Thermo Scientific LTQ Orbitrap 混合 FT 质谱仪和数据系统。该仪器直接连接到提供 LC/MS 和 (MS)n 功能的 Dionex Nano HPLC 系统，并将位于石溪医学院的蛋白质组学中心。该系统将提供高分辨率、精确的质量测量和电子转移解离，这两种新的质谱仪技术将显着促进构成主要用户组的石溪大学 (SBU) 13 名杰出科学家的研究目标和实验室生产力。这些方法目前在 SBU 中不可用，并且无法从其他组织获得或难以获得。 LC/MS/MS 在表征具有重要生物医学意义的分子方面具有无与伦比的优势，包括脂质、生物聚合物（如肽、寡核苷酸和寡糖）以及非挥发性、不稳定或热不稳定的较小化合物。通过电喷雾或大气压化学电离源将液相色谱仪与质谱仪耦合的技术现在可以轻松快速地对这些物质进行常规分析。准确质量数据的收集将有助于蛋白质和小分子表征，而 ETD 生成的碎片离子的质量分析将使蛋白质翻译后修饰的更完整表征成为可能。新的信息相关数据采集例程允许在单次注射期间对多种化合物进行完整分析。采用灵敏的 LC/MS/MS 和 iTRAQ 技术的定量方法对于一些研究人员来说至关重要。该仪器将由蛋白质组学中心经过培训的人员操作和维护，主任将安排日常操作并为用户提供技术专业知识。咨询委员会将确保与主要用户和其他 PHS 支持的研究人员接触，并将负责仪器的长期运行。公共卫生相关性创新的生物医学研究需要经常使用最新的分析技术，以增进对人类疾病的了解并为这些疾病提供新的治疗方法。 Thermo Scientific LTQ Orbitrap 混合 FT 质谱仪和数据系统将允许对许多生物分子进行低水平分析，这些生物分子对于 NIH 支持的石溪研究人员的研究工作至关重要。他们将使用该仪器提供的新分析技术（精确质量测量和电子转移解离）来探索导致糖尿病、癌症和心脏病的机制，并帮助定义针对这些疾病状态的新生化标记物和药物。

项目成果

期刊论文数量（3）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

A nitric oxide-responsive quorum sensing circuit in Vibrio harveyi regulates flagella production and biofilm formation.

DOI：
10.3390/ijms140816473
发表时间：
2013-08-08
期刊：
International journal of molecular sciences
影响因子：
5.6
作者：
Henares BM;Xu Y;Boon EM
通讯作者：
Boon EM

Detecting the immune system response of a 500 year-old Inca mummy.

DOI：
10.1371/journal.pone.0041244
发表时间：
2012
期刊：
PloS one
影响因子：
3.7
作者：
Corthals A;Koller A;Martin DW;Rieger R;Chen EI;Bernaski M;Recagno G;Dávalos LM
通讯作者：
Dávalos LM

Factors influencing the assimilation of arsenic in a deposit-feeding polychaete.