Acquisition of an Applied Biosystems 4000 QTRAP Mass Spectrometer

购买 Applied Biosystems 4000 QTRAP 质谱仪

• 批准号: 7214942
• 负责人: CHARLES R IDEN
• 金额: $ 43.46万
• 依托单位: STATE UNIVERSITY NEW YORK STONY BROOK
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-03-01 至 2008-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7214942
• 关键词: Acquisition; Applied; Biosystems; 4000; QTRAP

DESCRIPTION (provided by applicant): This proposal requests funds for the purchase of an Applied Biosystems 4000 QTRAP Mass Spectrometer and Data System. The instrument is linked directly to an Eksigent nano HPLC system affording LC/MS/MS capability and will be located in the Proteomics Center in the Stony Brook School of Medicine. Demand has now exceeded the ability to perform MS/MS experiments in the Center. This system will address this critical problem and will significantly promote the research objectives and laboratory productivity of eight distinguished scientists from Stony Brook University (SBU) who comprise the major users group. Additional biomedical research investigators will also have access as instrument. This mass spectrometer system will make directly available to these investigators important new techniques for the analysis of biomolecules, which are presently unavailable at SBU and inaccessible or difficult to obtain from other organizations. LC/MS/MS is unsurpassed for the characterization of molecules with a vital biomedical significance, including lipids, biopolymers such as peptides, oligonucleotides, and oligosaccharides and smaller compounds which are non- volatile, labile, or thermally unstable. Techniques for coupling a liquid chromatograph to a mass spectrometer through electrospray or atmospheric pressure chemical ionization sources now permit the routine analysis of these substances with ease and rapidity. New information dependent data acquisition routines permit full characterization of multiple compounds during a single injection. Quantitative methods employing sensitive LC/MS/MS techniques are essential for several investigators. The instrument will be operated and maintained by trained personnel at the Proteomics Center, and the Director will schedule daily operation and provide technical expertise for the users. An advisory committee will ensure access to the major users and other PHS- supported investigators and will be responsible for the long term operation of the instrument. Relevance Innovative biomedical research requires frequent access to the newest analytical techniques to advance knowledge of human diseases and to provide new treatments for these illnesses. The Applied Biosystems 4000 QTRAP instrument will permit low-level analysis of many biomolecules critical to the research efforts of NIH-supported investigators at Stony Brook. They will use the new, analytical techniques offered by this instrument to explore mechanisms leading to diabetes, cancer, and heart disease and help define new biochemical markers and medications for each disease state.

描述（由申请人提供）：该提案要求购买Applied Biosystems 4000 QTRAP质谱仪和数据系统的资金。该仪器直接链接到具有LC/MS/MS功能的Eksigent Nano HPLC系统，并将位于Stony Brook医学院的蛋白质组学中心。现在，需求已超出了中心执行MS/MS实验的能力。该系统将解决这个关键问题，并将大大促进来自主要用户组的Stony Brook University（SBU）的八位杰出科学家的研究目标和实验室生产力。其他生物医学研究人员也将作为工具访问。该质谱仪系统将直接向这些研究者提供重要的新技术，以分析生物分子，这些技术目前在SBU中无法获得，并且无法从其他组织那里获得或难以获得。 LC/MS/MS对于具有重要生物医学意义的分子的表征，包括脂质，肽，寡核苷酸，寡核苷酸和寡糖和较小的化合物，这些分子具有重要的生物医学意义，而LC/MS/MS无与伦比。现在，通过电喷雾或大气压化学电离源将液相色谱仪与质谱仪耦合的技术现在可以轻松且迅速地对这些物质进行常规分析。新信息依赖的数据采集例程允许在单个注射过程中对多种化合物进行全面表征。使用敏感LC/MS/MS技术的定量方法对于几个研究人员至关重要。该仪器将由蛋白质组学中心训练有素的人员进行操作和维护，主任将安排日常操作并为用户提供技术专业知识。咨询委员会将确保访问主要用户和其他受支持的调查人员，并负责该工具的长期操作。相关性创新的生物医学研究需要经常使用最新的分析技术，以提高人们对人类疾病的了解并为这些疾病提供新的治疗方法。 Applied Biosystems 4000 QTRAP仪器将允许对许多对NIH支持的研究人员在Stony Brook的研究工作至关重要的生物分子的低级分析。他们将使用该工具提供的新的分析技术来探索导致糖尿病，癌症和心脏病的机制，并有助于为每种疾病状态定义新的生化标记和药物。

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