SYNTHETIC & ANALYTICAL

合成的

基本信息

批准号：
8069938
负责人：
CHARLES R IDEN
金额：
$ 32.23万
依托单位：
STATE UNIVERSITY NEW YORK STONY BROOK
依托单位国家：
美国
项目类别：
财政年份：
2010
资助国家：
美国
起止时间：
2010-05-05 至 2012-04-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8069938
关键词：
2&apos -deoxyadenosine Aristolochic Acids Arts Biological Cells Chemicals Core Facility DNA DNA biosynthesis Detection Development Fluorescence High Pressure Liquid Chromatography Human Resources Improve Access Individual Inorganic Sulfates Investigation Kidney Kidney Diseases Maps Mass Spectrum Analysis Methods Mus National Center for Research Resources Peptides Progress Reports Proteins Proteomics Rattus Relative (related person)Reporting Research Research Personnel Resources Rodent Model Sampling Schedule Services Site Source Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Study Section System Techniques Technology Testing Time United States National Institutes of Health Unspecified or Sulfate Ion Sulfates Work adduct analog analytical method aristolochic acid I aristolochic acid II chemical synthesis detector experience instrument instrumentation mass spectrometer meetings metabolic abnormality assessment novel phosphoramidite programs

项目摘要

a pure sample as a standard. New peptide adducts formed by AL-II have been isolated by HPLC using a fluorescence detector and analyzed by MALDI mass spectrometry. A brief report of each of these advances is found in the Progress Report/Preliminary Studies section. Finally, Dr. Iden, seeking to improve access to state-of-the-art mass spectrometry techniques, submitted a proposal for a new triple quadrupole mass spectrometer system to the NIH National Center for Research Resources. Council is scheduled to meet in October, 2006. If this instrument is acquired in the Spring, 2007, as scheduled, levels of sensitivity for quantitative detection of DNA and protein adducts will be increased by a factor of ten over that attained by our current triple quadrupole instrumentation. A. Specific Aims Oligodeoxynucleotide synthesis and chemical analysis performed by the Core Facility provides fundamental support for all Program research. The Core will provide modified and normal oligomers to all investigators. DNA synthesis personnel will work closely with synthetic chemists to test new, modified deoxynucleosides phosphoramidites and incorporate them into DNA oligomers. We will refine our quantitative mass spectrometer methods and proteomics capabilities and focus on the development of sensitive, new techniques to identify DNA and protein adducts formed by the aristolochic acids (AAs) and their metabolites. The specific aims of the Core Facility are: 1. Toprepare

一个纯样品作为标准。由Al-II形成的新肽加合物已通过HPLC分离荧光检测器并通过MALDI质谱法分析。这些进步的简短报告是在“进度报告/初步研究”部分中找到。最后，伊登博士，试图改善获得的机会最先进的质谱技术，提交了针对新三杆质量的提案光谱仪系统到NIH国家研究资源中心。理事会定于2006年10月开会。如果该工具是在2007年春季获得的按照计划，通过a增加了对DNA和蛋白质加合物定量检测的敏感性水平我们当前三倍四极杆仪器所获得的十倍。 A.具体目标核心设施进行的寡脱氧核苷酸合成和化学分析提供了基本支持所有计划研究。核心将为所有研究人员提供改良和正常的低聚物。 DNA合成人员将与合成化学家紧密合作，以测试新的，改良的脱氧核苷磷光胺并将其掺入DNA低聚物中。我们将完善定量质谱仪方法和蛋白质组学功能，并着重于开发敏感的新技术来识别由aristolochic酸（AAS）及其代谢产物形成的DNA和蛋白质加合物。核心设施的具体目的是： 1。Toprepare