CORE--SCIENTIFIC

核心--科学

• 批准号: 6443875
• 负责人: CHARLES R IDEN
• 金额: $ 21.9万
• 依托单位: STATE UNIVERSITY NEW YORK STONY BROOK
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-04-01 至 2002-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6443875
• 关键词: analytical chemistry; biomedical facility; high performance liquid chromatography; mass spectrometry; nucleic acid chemical synthesis; oligonucleotides

The objectives of the core component are three-fold: (1) to provide administrative support for participants in the Program; (2) to establish centralized facilities for chemical analysis and oligodeoxynucleotide synthesis; and (3) to conduct a seminar series in which visiting scientists will be invited to discuss their research with Program members. The administrative structure of the Program is substantially unchanged. The Core Project has been expanded to include sophisticated mass spectrometer analysis as well as automated DNA synthesis and purification. Mass spectrometry will play a critical role in the analysis of adducts and synthetic intermediates; we hope to add an important new component, a LC/MS thermospray system, to our current facilities. Synthesis of oligodeoxynucleotides is essential for all investigators in the Program; this can most effectively be accomplished by a centralized service facility. Synthesis of certain chemically-modified oligodeoxynucleotides will require large scale preparation of modified deoxynucleoside phosphoramidites. This will be conducted in the core facility.

核心组件的目标是三倍：（1）提供 对该计划的参与者的行政支持； （2）建立 化学分析和寡脱氧核苷酸的集中设施 合成; （3）进行一个研讨会系列，在其中访问科学家 将邀请与计划成员讨论他们的研究。 该计划的管理结构基本不变。这 核心项目已扩展到包括复杂的质谱仪 分析以及自动DNA合成和纯化。大量的 光谱法将在加合物的分析中起关键作用 合成中间体；我们希望添加一个重要的新组件，一个LC/MS 热喷系统，我们当前的设施。 寡脱氧核苷酸的合成对于所有研究者至关重要 该计划；这可以通过集中式来实现 服务设施。某些化学修饰的合成 寡脱氧核苷酸将需要大规模制备改良的 脱氧核苷磷酰胺。这将在核心中进行 设施。