CHARACTERIZATION OF PARASYMPATHETIC GANGLIA

副交感神经节的特征

• 批准号: 3396756
• 负责人: PATRICIA SHINNICK-GALLAGHER
• 金额: $ 12.75万
• 依托单位: UNIVERSITY OF TEXAS MED BR GALVESTON
• 依托单位国家: 美国
• 财政年份: 1980
• 资助国家: 美国
• 起止时间: 1980-04-01 至 1987-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3396756
• 关键词: antiadrenergic agents; antidromic impulse; autonomic ganglion; cell cell interaction; electrical potential; electrophysiology; fluorescent dye /probe; innervation; interference microscopy; neural plasticity; neural transmission; neuroanatomy; neurotransmitters; nontherapeutic iontophoresis; parasympathetic nervous system; synapses; urinary bladder

The proposed research is designed to obtain a basic understanding of the electrophysiology, pharmacology and morphology of mammalian parasympathetic ganglia and to define the local neuronal interactions which contribute to the integrative function of autonomic ganglia, in general. Local neuronal interactions which occur during ganglionic transmission will be analyzed in cat vesical pelvic ganglia (VPG) using intracellular, single electrode voltage clamp, and iontophoretic techniques coupled with differential interference microscopy. The following local neuronal interactions will be investigated in mammalian parasympathetic ganglia 1) the muscarinic inhibitory and excitatory slow synaptic potentials 2) the late slow modulatory potentials 3) synaptic events underlying synaptic plasticity, 4) intrinsic cell-to-cell communication and 5) the neurotransmitter/neuromodulatory role of leucine-enkephalin. The sites, mechanisms and functional significance of the local neuronal interactions which occur in bladder ganglia will be determined. Analysis of the mechanisms underlying these local neuronal interactions may lead to a better understanding of autonomic function, in general, as well as "higher" brain functions involved in memory, learning, and behavior. In addition, the information obtained from pharmacologicaly analysis of these synaptic mechanisms may eventually result in use of drugs which are more clinically effective in the treatment of CNS disorders, in particular, Parkinson's Disease as well as autonomic dysfunction, especially, bladder dysfunction.

拟议的研究旨在获得对 哺乳动物副交感神经的电生理学、药理学和形态学 神经节并定义局部神经元相互作用，这有助于 一般而言，自主神经节的整合功能。 局部神经元 神经节传递过程中发生的相互作用将被分析 使用细胞内单电极测量猫膀胱盆腔神经节 (VPG) 电压钳和离子电渗技术与微分相结合 干涉显微镜。 以下局部神经元相互作用将是 在哺乳动物副交感神经节中进行研究 1) 毒蕈碱 抑制性和兴奋性慢突触电位 2) 晚期慢 调节电位 3) 突触可塑性背后的突触事件，4) 内在的细胞间通讯和5） 亮氨酸脑啡肽的神经递质/神经调节作用。 这些网站， 局部神经元相互作用的机制和功能意义 哪些发生在膀胱神经节将被确定。 分析 这些局部神经元相互作用的潜在机制可能会导致 一般来说，更好地理解自主功能以及“更高” 大脑功能涉及记忆、学习和行为。 此外， 从这些突触的药理学分析中获得的信息 机制可能最终导致使用更临床化的药物 有效治疗中枢神经系统疾病，特别是帕金森病 疾病以及植物神经功能障碍，尤其是膀胱功能障碍。