CHARACTERIZATION OF PARASYMPATHETIC GANGLIA

副交感神经节的特征

• 批准号: 3396756
• 负责人: PATRICIA SHINNICK-GALLAGHER
• 金额: $ 12.75万
• 依托单位: UNIVERSITY OF TEXAS MED BR GALVESTON
• 依托单位国家: 美国
• 财政年份: 1980
• 资助国家: 美国
• 起止时间: 1980-04-01 至 1987-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3396756
• 关键词: antiadrenergic agents; antidromic impulse; autonomic ganglion; cell cell interaction; electrical potential; electrophysiology; fluorescent dye /probe; innervation; interference microscopy; neural plasticity; neural transmission; neuroanatomy; neurotransmitters; nontherapeutic iontophoresis; parasympathetic nervous system; synapses; urinary bladder

The proposed research is designed to obtain a basic understanding of the electrophysiology, pharmacology and morphology of mammalian parasympathetic ganglia and to define the local neuronal interactions which contribute to the integrative function of autonomic ganglia, in general. Local neuronal interactions which occur during ganglionic transmission will be analyzed in cat vesical pelvic ganglia (VPG) using intracellular, single electrode voltage clamp, and iontophoretic techniques coupled with differential interference microscopy. The following local neuronal interactions will be investigated in mammalian parasympathetic ganglia 1) the muscarinic inhibitory and excitatory slow synaptic potentials 2) the late slow modulatory potentials 3) synaptic events underlying synaptic plasticity, 4) intrinsic cell-to-cell communication and 5) the neurotransmitter/neuromodulatory role of leucine-enkephalin. The sites, mechanisms and functional significance of the local neuronal interactions which occur in bladder ganglia will be determined. Analysis of the mechanisms underlying these local neuronal interactions may lead to a better understanding of autonomic function, in general, as well as "higher" brain functions involved in memory, learning, and behavior. In addition, the information obtained from pharmacologicaly analysis of these synaptic mechanisms may eventually result in use of drugs which are more clinically effective in the treatment of CNS disorders, in particular, Parkinson's Disease as well as autonomic dysfunction, especially, bladder dysfunction.

拟议的研究旨在获得对 哺乳动物副交感神经的电生理学，药理学和形态 神经节并定义有助于局部神经元相互作用 通常，自主神经节的综合功能。 局部神经元 在神经节传播过程中发生的相互作用将在 使用细胞内的单电极，猫囊泡骨盆神经节（VPG） 电压夹和离子电池技术和差分 干扰显微镜。 以下局部神经元相互作用将是 在哺乳动物副交感神经节中进行了研究1）毒蕈碱 抑制性和兴奋性缓慢的突触电位2）晚期慢 调节电位3）突触可塑性的突触事件，4） 固有的单元间通信和5） 亮氨酸 - 源自源自神经递质/神经调节作用。 网站， 局部神经元相互作用的机制和功能意义 将确定在膀胱神经节中发生的。 分析 这些局部神经元相互作用的机制可能导致 一般来说，更好地了解自主功能以及“更高” 记忆，学习和行为涉及的大脑功能。 此外， 从这些突触的药理分析获得的信息 机制最终可能导致使用更临床的药物 有效治疗中枢神经系统疾病，尤其是帕金森氏症 疾病以及自主神经功能障碍，尤其是膀胱功能障碍。