CHARACTERIZATION OF PARASYMPATHETIC GANGLIA

副交感神经节的特征

基本信息

批准号：
3396758
负责人：
PATRICIA SHINNICK-GALLAGHER
金额：
$ 10.6万
依托单位：
UNIVERSITY OF TEXAS MED BR GALVESTON
依托单位国家：
美国
项目类别：
财政年份：
1980
资助国家：
美国
起止时间：
1980-04-01 至 1991-03-31
项目状态：
已结题

项目摘要

The long term objective of this research is to characterize transmission through cat bladder parasympathetic ganglia and thereby to define the local neuronal interactions which may contribute to the integrative function of autonomic ganglia and to higher CNS functions involved in memory and learning. Information derived from this research would enhance our understanding of the neural control of the urinary bladder and its physiology and pharmacology. Local neuronal interactions which occur during ganglionic transmission will be analyzed in cat parasympathetic ganglia of the urinary bladder using intracellular, single and double electrode voltage clamp and single channel- patch clamp analysis. The local neuronal interactions which will be analyzed in this proposal are endogenously occurring synaptic events mediated by three different neurotransmitters, namely, acetylcholine (ACh), norepinephrine (NE) and adenosine. Evidence suggests that all three of these endogenous neurotransmitter mediated hyperpolarizing synaptic potentials are mediated by a calcium dependent potassium conductance. The outward K currents underlying the sow inhibitory synaptic potentials in cat bladder parasympathetic ganglia will be analyzed as well as pharmacological charcteristics of and possible second messenger systems underlying the synaptic responses mediated by the neurotransmitters, acetylcholine, norepinephrine, and adenosine. In addition the membrane K channels activated by ACh, NE, and adenosine wil be compared with the properties of Ca-activated K channels located in the membranes of cat bladder parasympathic neurons. The above studies should provide essential information about the membrane mechanisms involved in neuronal communication between pre- and post-synaptic neurons, and about possible mechanisms linking electrophysiological events with intracellular second messengers and cell metabolism. Analysis of the mechanisms underlying these local neuronal interactions may lead to a better understanding of autonomic function, as well as "higher" brain functions involved in memory, learning, and behavior. In addition, the information obtained form pharmacological analysis of these synaptic mechanisms may eventually result in the use of drugs which are more clinically effective in the treatment of CNS disorders, as well as autonomic dysfunction, particularly, bladder dysfunction.

这项研究的长期目标是表征通过猫膀胱副交感神经节传播从而定义局部神经元相互作用有助于自主神经节的整合功能涉及记忆和学习的高级中枢神经系统功能。从这项研究中获得的信息将增强我们的了解膀胱及其神经控制生理学和药理学。局部神经元相互作用神经节传输期间发生的情况将在 cat 中进行分析膀胱副交感神经节使用细胞内，单、双电极电压钳和单通道膜片钳分析。本节将分析局部神经元相互作用建议是内源性发生的突触事件介导三种不同的神经递质，即乙酰胆碱（ACh），去甲肾上腺素（NE）和腺苷。有证据表明，所有其中三种内源性神经递质介导超极化突触电位由钙介导依赖性钾电导。母猪抑制突触下的向外 K 电流将分析猫膀胱副交感神经节的电位以及药理特征和可能的第二个突触反应背后的信使系统神经递质、乙酰胆碱、去甲肾上腺素和腺苷。此外，膜 K 通道被激活 ACh、NE 和腺苷将与位于猫膀胱膜的钙激活钾离子通道副交感神经元。上述研究应提供有关以下方面的重要信息：参与神经元通讯的膜机制突触前和突触后神经元之间，以及大约可能的将电生理事件与细胞内联系起来的机制第二信使和细胞代谢。分析这些局部神经元相互作用的潜在机制可能导致更好地理解自主功能，以及 “高级”大脑功能涉及记忆、学习和行为。此外，从表格中获取的信息这些突触机制的药理学分析可能最终导致使用更适合临床的药物有效治疗中枢神经系统疾病以及自主神经疾病功能障碍，特别是膀胱功能障碍。