CHARACTERIZATION OF PARASYMPATHETIC

副交感神经的特征

• 批准号: 3396755
• 负责人: PATRICIA SHINNICK-GALLAGHER
• 金额: $ 13.83万
• 依托单位: UNIVERSITY OF TEXAS MED BR GALVESTON
• 依托单位国家: 美国
• 财政年份: 1980
• 资助国家: 美国
• 起止时间: 1980-04-01 至 1990-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3396755
• 关键词: adenosine; antiadrenergic agents; antidromic impulse; autonomic ganglion; cell cell interaction; electrical potential; electrophysiology; fluorescent dye /probe; innervation; interference microscopy; neural plasticity; neural transmission; neuroanatomy; neurotransmitters; nontherapeutic iontophoresis; norepinephrine; parasympathetic nervous system; synapses; urinary bladder

The long term objective of this research is to characterize transmission through cat bladder parasympathetic ganglia and thereby to define the local neuronal interactions which may contribute to the integrative function of autonomic ganglia and to higher CNS functions involved in memory and learning. Information derived from this research would enhance our understanding of the neural control of the urinary bladder and its physiology and pharmacology. Local neuronal interactions which occur during ganglionic transmission will be analyzed in cat parasympathetic ganglia of the urinary bladder using intracellular, single and double electrode voltage clamp and single channel- patch clamp analysis. The local neuronal interactions which will be analyzed in this proposal are endogenously occurring synaptic events mediated by three different neurotransmitters, namely, acetylcholine (ACh), norepinephrine (NE) and adenosine. Evidence suggests that all three of these endogenous neurotransmitter mediated hyperpolarizing synaptic potentials are mediated by a calcium dependent potassium conductance. The outward K currents underlying the sow inhibitory synaptic potentials in cat bladder parasympathetic ganglia will be analyzed as well as pharmacological charcteristics of and possible second messenger systems underlying the synaptic responses mediated by the neurotransmitters, acetylcholine, norepinephrine, and adenosine. In addition the membrane K channels activated by ACh, NE, and adenosine wil be compared with the properties of Ca-activated K channels located in the membranes of cat bladder parasympathic neurons. The above studies should provide essential information about the membrane mechanisms involved in neuronal communication between pre- and post-synaptic neurons, and about possible mechanisms linking electrophysiological events with intracellular second messengers and cell metabolism. Analysis of the mechanisms underlying these local neuronal interactions may lead to a better understanding of autonomic function, as well as "higher" brain functions involved in memory, learning, and behavior. In addition, the information obtained form pharmacological analysis of these synaptic mechanisms may eventually result in the use of drugs which are more clinically effective in the treatment of CNS disorders, as well as autonomic dysfunction, particularly, bladder dysfunction.

这项研究的长期目标是表征 通过猫膀胱副交感神经的传播和 从而定义可能的局部神经元相互作用 有助于自主神经节的综合功能和 较高的中枢神经系统功能涉及记忆和学习。 从这项研究中得出的信息将增强我们的 了解膀胱的神经控制及其神经控制 生理学和药理学。 局部神经元相互作用 在CAT中将分析神经节传播期间发生 使用细胞内的膀胱副交感神经神经节 单电极电压夹和单个通道 - 斑块夹分析。 将在此分析的局部神经元相互作用 提案是由内源性发生的突触事件介导的 三种不同的神经递质，即乙酰胆碱（ACH）， 去甲肾上腺素（NE）和腺苷。 有证据表明所有 这些内源性神经递质介导的三个 超极化突触电位由钙介导 依赖性钾电导。 SOW抑制性突触的外部K电流 将分析猫膀胱副交感神经的潜力 以及可能第二 Messenger系统由由 神经递质，乙酰胆碱，去甲肾上腺素和 腺苷。 另外，膜K通道激活 将ACH，NE和腺苷与 CA激活的K通道位于猫膀胱膜上 副交感神经元。 以上研究应提供有关 涉及神经元通信的膜机制 在突触前和突触后神经元之间以及可能 将电生理事件与细胞内连接的机制 第二使者和细胞代谢。 分析 这些局部神经元相互作用的机制可能会导致 更好地了解自主功能以及 “更高”的大脑功能参与记忆，学习和 行为。 此外，获得的信息表格 这些突触机制的药理分析可能 最终导致使用更临床的药物 有效治疗中枢神经系统疾病以及自主神经 功能障碍，特别是膀胱功能障碍。