AMYGDALA NEURONS AND FEAR CONDITIONING

杏仁核神经元和恐惧条件反射

• 批准号: 6528495
• 负责人: PATRICIA SHINNICK-GALLAGHER
• 金额: $ 18.2万
• 依托单位: UNIVERSITY OF TEXAS MED BR GALVESTON
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-08-01 至 2005-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6528495
• 关键词: NMDA receptors; action potentials; amygdala; behavioral /social science research tag; biocytin; brain electrical activity; cell type; conditioning; electrostimulus; fear; glutamate receptor; laboratory rat; membrane potentials; neural transmission; synapses; voltage /patch clamp; NMDA receptors; action potentials; amygdala; behavioral /social science research tag; biocytin; brain electrical activity; cell type; conditioning; electrostimulus; fear; glutamate receptor; laboratory rat; membrane potentials; neural transmission; synapses; voltage /patch clamp

DESCRIPTION (adapted from applicant's abstract): The amygdala is known to play a critical role in emotional responses particularly fear, in both humans and animals. The amygdala and its afferent and efferent connections comprise a major component of the auditory fear conditioning circuitry. The long-term objective of this research is to characterize pre- and postsynaptic modifications in amygdala glutamatergic neurotransmission underlying the expression of learned fear. Preliminary data show significant alterations in synaptic transmission in the internal capsule (IC) fiber pathway from the medial geniculate to the dorsal lateral amygdala recorded in vitro in amygdala slices from paired fear conditioned but not unpaired control animals. The proposed experiments using the fear-potentiated startle paradigm will test the hypothesis that lasting potentiation of synaptic transmission occurs at particular synapses within the fear conditioning intraamygdala circuitry. The following specific aims will be addressed using whole cell patch recording in amygdala slice preparations from three populations of animals. naive control, unpaired control and paired fear conditioned animals: 1) Characterize the modifications in synaptic transmission and membrane conductance underlying fear conditioning and determine the pre- and post-synaptic changes in N-methyl-D-aspartate (NMDA)- and non-NMDA-mediated synaptic transmission in animals exposed to a paired conditioned stimulus (CS) and aversive stimulus (UCS) with those exposed to the same information but in an unpaired paradigm and 2) trace the information flow through the amygdala by comparing in the three populations of animals the synaptic modifications occurring in glutamatergic transmission at different synapses in the amygdala fear conditioning circuitry. The results of the proposed experiments will enhance our understanding of the membrane mechanisms underlying emotional learning at the membrane and whole cell level and provide important information about changes in the essential elements of interneuronal communication within a key structure involved in emotion, the amygdala. Ultimately the proposed studies may provide insight into potential therapeutic strategies in the treatment of neuropsychiatric disorders such as anxiety, phobia, schizophrenia and in particular posttraumatic stress disorder.

描述（根据申请人的摘要改编）：已知杏仁核 在情感反应中起着至关重要的作用，尤其是恐惧 人类和动物。 杏仁核及其传入和传出连接 包括听觉恐惧调节电路的主要组成部分。 这 这项研究的长期目标是表征前和 杏仁核神经传递的突触后修饰 基于学习恐惧的表达。 初步数据显示 内囊突触传播的重大改变 （IC）纤维途径从内侧元素到背侧杏仁核 从配对的恐惧中记录在杏仁核切片中的体外记录，但没有 未配对的对照动物。 提出的实验使用 恐惧态度的惊吓范式将检验持久的假设 突触传播的增强发生在特定的突触中 恐惧调节腔内电路。 以下特定目标 将使用Amygdala Slice中的全单元格记录来解决 来自三个动物种群的制剂。 幼稚的控制，不配对 控制和配对的恐惧条件动物：1）表征 突触传播和膜电导的修改 恐惧调节并确定前后突触后的变化 N-甲基-D-天冬氨酸（NMDA） - 和非NMDA介导的突触传播 暴露于配对条件刺激（CS）和厌恶刺激的动物 （UCS）带有相同信息的那些，但在未配对的范式中 2）通过比较，跟踪信息流过杏仁核的信息 三个动物种群发生的突触修饰发生在 杏仁核恐惧中不同突触的谷氨酸能传播 调理电路。 提出的实验的结果将 增强我们对情感膜机制的理解 在膜和整个细胞水平上学习，并提供重要的 有关神经元中基本要素的变化的信息 情感涉及的关键结构内的沟通，杏仁核。 最终，拟议的研究可能会洞悉潜在的 治疗神经精神疾病的治疗策略 作为焦虑，恐惧症，精神分裂症，尤其是创伤后压力 紊乱。