17ALERT bid: A new multi-wavelength analytical ultracentrifuge for the study of biomolecular interactions

17ALERT bid：用于研究生物分子相互作用的新型多波长分析超速离心机

• 批准号: BB/R013411/1
• 负责人: David Scott
• 金额: $ 52.57万
• 依托单位: University of Nottingham
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2018
• 资助国家: 英国
• 起止时间: 2018 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FR013411%2F1
• 关键词: 17ALERT; bid; new; multi; wavelength

An analytical ultracentrifuge (AUC) is a machine capable of observing macromolecules sediment under gravity when a rotor is spun at a speeds from 1000 to 60 000 rpm. We are able to observe their sedimentation properties due to optical data capture systems mounted on the instrument. To date, these have be limited to single wavelength capture and an allied Rayleigh Interference optical system. A new AUC released by Beckman Coulter is now capable of true multi-wavelength capture so that more complex biomolecular systems can be analysed by following each component due to its unique optical signature. Where there is no unique optical signature, it is possible to label a compound either with a coloured probe, or incorporate into a protein using analogues of natural amino acids. As such, the instrument is capable of measuring a very wide range of biomolecular interactions and characterising them rigorously. In addition, the instrument will be maintained by a permanent technician, data analysis will be provided by the PI who is an expert in AUC, and new software methods will be provided through workshops. The latter is provided by a grant from the Fullbright Fund, which will allow a week long workshop to be undertaken. Given the wide range of systems that can be worked on to give information about size, shape and stoichiometry of biomolecules, this application ranges widely across BBSRC priorities and strategies.

分析超速离心机 (AUC) 是一种当转子以 1000 至 60 000 rpm 的速度旋转时能够在重力作用下观察大分子沉积物的机器。由于仪器上安装了光学数据采集系统，我们能够观察它们的沉降特性。迄今为止，这些仅限于单波长捕获和联合瑞利干涉光学系统。 Beckman Coulter 发布的新 AUC 现在能够实现真正的多波长捕获，因此可以通过跟踪每个组件（由于其独特的光学特征）来分析更复杂的生物分子系统。如果没有独特的光学特征，则可以使用有色探针标记化合物，或使用天然氨基酸类似物掺入蛋白质中。因此，该仪器能够测量非常广泛的生物分子相互作用并严格表征它们。此外，仪器将由常驻技术人员维护，数据分析将由AUC专家PI提供，新的软件方法将通过研讨会提供。后者由 Fullbright 基金提供赠款，这将允许举办为期一周的研讨会。鉴于可用于提供有关生物分子的尺寸、形状和化学计量信息的系统范围广泛，该应用广泛涵盖了 BBSRC 的优先事项和战略。

项目成果

A super-potent tetramerized ACE2 protein displays enhanced neutralization of SARS-CoV-2 virus infection.

• DOI: 10.1038/s41598-021-89957-z
• 发表时间: 2021-05-19
• 期刊: Scientific reports
• 影响因子: 4.6
• 作者: Miller A;Leach A;Thomas J;McAndrew C;Bentley E;Mattiuzzo G;John L;Mirazimi A;Harris G;Gamage N;Carr S;Ali H;Van Montfort R;Rabbitts T
• 通讯作者: Rabbitts T

Structure-guided enhancement of selectivity of chemical probe inhibitors targeting bacterial seryl-tRNA synthetase

• DOI: 10.1101/586255
• 发表时间: 2019-03
• 期刊: bioRxiv
• 作者: Ricky Cain;R. Salimraj;A. Punekar;D. Bellini;C. Fishwick;L. Czaplewski;D. Scott;G. Harris;C. Dowson;A. Lloyd;D. Roper

Schistosoma mansoni cathepsin D1: Biochemical and biophysical characterization of the recombinant enzyme expressed in HEK293T cells

• DOI: 10.1016/j.pep.2019.105532
• 发表时间: 2020-03-01
• 期刊: PROTEIN EXPRESSION AND PURIFICATION
• 影响因子: 1.6
• 作者: Araujo-Montoya, B. O.;Senger, M. R.;Silva-Jr, F. P.
• 通讯作者: Silva-Jr, F. P.

Structural modeling of an outer membrane electron conduit from a metal-reducing bacterium suggests electron transfer via periplasmic redox partners.

• DOI: 10.1074/jbc.ra118.001850
• 发表时间: 2018-05-25
• 期刊: The Journal of biological chemistry
• 作者: Edwards MJ;White GF;Lockwood CW;Lawes MC;Martel A;Harris G;Scott DJ;Richardson DJ;Butt JN;Clarke TA
• 通讯作者: Clarke TA

The structure of nontypeable Haemophilus influenzae SapA in a closed conformation reveals a constricted ligand-binding cavity and a novel RNA binding motif.

• DOI: 10.1371/journal.pone.0256070
• 发表时间: 2021
• 期刊: PloS one
• 影响因子: 3.7
• 作者: Lukacik P;Owen CD;Harris G;Bolla JR;Picaud S;Alibay I;Nettleship JE;Bird LE;Owens RJ;Biggin PC;Filippakopoulos P;Robinson CV;Walsh MA
• 通讯作者: Walsh MA