REGULATION OF SUGAR TRANSPORT AND METABOLISM IN LACTIC ACID AND ORAL BACTERIA

乳酸和口腔细菌中糖运输和代谢的调节

• 批准号: 2572317
• 负责人: J THOMPSON
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF DENTAL & CRANIOFACIAL RESEARCH
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/2572317
• 关键词: Fusobacterium; Lactobacillus; O glycosidase; Streptococcus; bacterial genetics; bacterial proteins; carbohydrate metabolism; carbohydrate transport; genetic markers; genetic promoter element; hydrolase; lactates; maltose; microorganism culture; microorganism metabolism; molecular cloning; nucleic acid sequence; oral bacteria; plasmids; sugar phosphates; transfection; transposon /insertion element

Microorganisms such as the Gram-positive Streptococci and Gram- negative Fusobacteria are causative or contributory agents in the etiology of dental caries, gingivitis and periodontal disease(s). The pathogenicity of these bacteria resides in part in their capacity to form a variety of organic acids and toxic sulfur-containing derivatives as end-products of carbohydrate and amino acid fermentation. These metabolic products are potentially cytotoxic for epithelial and other tissue cells. The aims of this research program are two-fold: (1) to identify and characterize the enzymes that constitute these energy-yielding pathways, and (2) to isolate genes encoding specific metabolic traits in these pathways for use as selectable markers for plasmid or transposon-mediated gene transfer in Fusobacteria. To this end, we have recently purified and characterized two unique phospho-glucosidases from Fusobacterium mortiferum ATCC 25557. The enzymes in question, maltose 6-phosphate hydrolase and phospho-beta-glucosidase, catalyze the hydrolysis of chemically synthesized chromogenic and fluorogenic alpha- and beta- glucoside 6-phosphates. The genes encoding the two enzymes, malH and pbgA respectively, have now been cloned and sequenced. When incorporated behind suitable promoters in an appropriate vector, these genes will be eminently suitable as selection markers for the genetic manipulation and analysis of virulence factors of pathogenic oral Fusobacteria.

微生物，例如革兰氏阳性链球菌和革兰氏阳性链球菌 阴性梭杆菌是致病菌或促成菌 龋齿、牙龈炎和牙周病的病因。 这些细菌的致病性部分取决于它们的能力 形成多种有机酸和有毒的含硫物质 作为碳水化合物和氨基酸最终产品的衍生物 发酵。 这些代谢产物具有潜在的细胞毒性 上皮细胞和其他组织细胞。 本研究计划的目的 有两个方面：（1）识别和表征酶 构成这些能量产生途径，以及（2）分离基因 在这些途径中编码特定的代谢特征，用作 用于质粒或转座子介导的基因转移的选择标记 在梭杆菌中。 为此，我们最近纯化并 鉴定了来自梭杆菌的两种独特的磷酸葡萄糖苷酶 mortiferum ATCC 25557。所讨论的酶，麦芽糖 6-磷酸 水解酶和磷酸-β-葡萄糖苷酶，催化水解 化学合成显色和荧光α-和β- 葡萄糖苷6-磷酸酯。 编码两种酶 malH 和 pbgA 分别已被克隆并测序。 什么时候 掺入适当载体中的适当启动子后面， 这些基因将非常适合作为选择标记 病原菌的基因操作及毒力因子分析 口腔梭杆菌。