REGULATION OF SUGAR TRANSPORT AND METABOLISM IN LACTIC ACID BACTERIA

乳酸菌糖运输和代谢的调控

• 批准号: 3963725
• 负责人: J THOMPSON
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF DENTAL & CRANIOFACIAL RESEARCH
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3963725
• 关键词: Fusobacterium; Lactobacillaceae; Lactobacillus casei; Streptococcus lactis; anaerobic bacteria; antibacterial agents; bioenergetics; carbohydrate metabolism; carbohydrate transport; deoxyglucose; fluorimetry; glucose analog; glucose transport; lysine; mass spectrometry; microorganism growth; microorganism immunology; microorganism metabolism; nuclear magnetic resonance spectroscopy; ornithine; peptide chemical synthesis; radiotracer

Two new amino acids have been discovered, isolated and purified from cells of Streptococcus lactis: N(5)-(1-carboxyethyl)ornithine and N(6)-(1-carboxyethyl) lysine. Confirmation of structure has been obtained by 1-H and 13-C nuclear magnetic resonance spectroscopy (NMR), mass spectroscopy and by chemical synthesis. Probable routes of in vivo synthesis of the two novel compounds, have been elucidated by radiotracer procedures and by thin-layer fluorography. The first detailed studies of sugar transport by the oral anaerobic microorganisms Peptostreptococcus anaerobius (Gram-positive) and Fusobacterium nucleatum (Gram-negative) have been conducted. Glucose transport by these bacteria is dependent upon the presence of specific amino acids in the extracellular environment. The biochemical basis for this unique amino acid dependency, and the modes of energy coupling to sugar accumulation have been established. The specificity of the glucose carrier in F. nucleatum has been determined by use of structural glucose analogs, and glycogen has been found to represent the major product of glucose uptake by the cell. These findings, obtained by use of [14-C]glucose and chemical analysis, have been elegantly confirmed by ultrastructural and morphological studies involving electron- and phase-contrast microscopy.

两种新氨基酸已从细胞中发现、分离和纯化 乳酸链球菌：N(5)-(1-羧乙基)鸟氨酸和 N(6)-(1-羧乙基)赖氨酸。 已获得结构确认 通过 1-H 和 13-C 核磁共振波谱 (NMR)，质量 光谱法和化学合成法。 体内可能的途径 放射性示踪剂阐明了这两种新型化合物的合成 程序和薄层荧光照相术。 首次详细研究 口腔厌氧微生物消化链球菌的糖转运 厌氧菌（革兰氏阳性）和具核梭杆菌（革兰氏阴性） 已进行。 这些细菌的葡萄糖转运取决于 细胞外环境中存在特定氨基酸。 这 这种独特的氨基酸依赖性的生化基础，以及 能量与糖积累的耦合已经建立。 这 F. nucleatum 中葡萄糖载体的特异性已通过以下方式确定 使用结构葡萄糖类似物和糖原已被发现代表 细胞摄取葡萄糖的主要产物。 这些发现，获得了 通过使用[14-C]葡萄糖和化学分析，已经优雅地 经涉及电子的超微结构和形态学研究证实 和相差显微镜。