REGULATION OF SUGAR TRANSPORT AND METABOLISM IN LACTIC ACID BACTERIA

乳酸菌糖运输和代谢的调控

• 批准号: 3939951
• 负责人: J THOMPSON
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF DENTAL & CRANIOFACIAL RESEARCH
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3939951
• 关键词: Fusobacterium; Lactobacillaceae; Lactobacillus casei; Streptococcus lactis; anaerobic bacteria; antibacterial agents; arginine; bioenergetics; carbohydrate metabolism; carbohydrate transport; deoxyglucose; fluorimetry; glucose analog; glucose transport; lysine; mass spectrometry; microorganism growth; microorganism immunology; microorganism metabolism; nuclear magnetic resonance spectroscopy; ornithine; peptide chemical synthesis; radiotracer; tissue /cell culture

A unique amino acid-dependent sugar transport system has been characterized in the Gram negative oral anaerobe, Fusobacterium nucleatum ATCC 10953. Energy necessary for the active transport of glucose and galactose is derived from anaerobic fermentation of specific amino acids (Glutamic, lysine, histidine). Kinetic analyses, and data from competition studies suggest that glucose and galactose are translocated via a common-affinity carrier. Intracellular sugars are phosphorylated by ATP- dependent kinases prior to conversion of high molecular-weight polymers. Glutamate, lysine and histidine prevent catabolism of the sugar polymers, but in the absence of amino acids the polymers are rapidly fermented to yield butyric, lactic and acetic acids. Two new amino acids have been identified and purified from Streptococcus lactis. These unusual amino acids, namely N(5)-(1- carboxyethyl)ornithine and N(6)-(1-carboxyethyl)lysine, have been chemically prepared and the stereochemical configurations of the natural products have been confirmed by chiral syntheses. The in vitro synthesis of N(5)-(1-carboxyethyl)-ornithine has been demonstrated by incubation of a cell-free extract with ornithine, pyruvic acid and NAD(P)H. A basic amino acid carrier has been identified and characterized in cells of Streptococcus lactis. The transporter mediates the energy-independent entry and exchange of lysine, arginine and ornithine.

一种独特的氨基酸依赖性糖转运系统 以革兰氏阴性口腔厌氧菌、梭杆菌为特征 nucleatum ATCC 10953。活性所需的能量 葡萄糖和半乳糖的转运来源于厌氧 特定氨基酸（谷氨酸、赖氨酸、组氨酸）的发酵。 动力学分析和竞争研究数据表明 葡萄糖和半乳糖通过共同亲和力易位 载体。 细胞内的糖被 ATP 磷酸化 高分子量转化之前的依赖性激酶 聚合物。 谷氨酸、赖氨酸和组氨酸可防止分解代谢 糖聚合物，但在没有氨基酸的情况下 聚合物快速发酵产生丁酸、乳酸和乙酸 酸。 已从其中鉴定并纯化了两种新氨基酸 乳酸链球菌。 这些不常见的氨基酸，即N(5)-(1- 羧乙基)鸟氨酸和N(6)-(1-羧乙基)赖氨酸，已被 化学制备和立体化学构型 天然产物已通过手性合成得到证实。 在 N(5)-(1-羧乙基)-鸟氨酸的体外合成 通过将无细胞提取物与鸟氨酸一起孵育来证明， 丙酮酸和 NAD(P)H。 碱性氨基酸载体已被鉴定和表征 在乳酸链球菌细胞中。 转运蛋白介导 赖氨酸、精氨酸和赖氨酸的能量独立进入和交换 鸟氨酸。