REGULATION OF SUGAR TRANSPORT AND METABOLISM IN LACTIC ACID BACTERIA

乳酸菌糖运输和代谢的调控

• 批准号: 4692641
• 负责人: J THOMPSON
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF DENTAL & CRANIOFACIAL RESEARCH
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/4692641
• 关键词: Lactobacillaceae; Lactobacillus casei; Streptococcus lactis; anaerobic bacteria; antibacterial agents; bioenergetics; carbohydrate metabolism; carbohydrate transport; deoxyglucose; glucose analog; microorganism growth; microorganism immunology; microorganism metabolism; radiotracer

A membrane-regulated, sugar-phosphate: phosphohydrolase has been purified and characterized from Lactobacillus casei. Antibodies to the enzyme have been obtained for use in structure/function experiments. Lactose analogs containing non-metabolizable, (14C)-labeled 2-fluoro-D-glucose or 2-deoxy-D-glucose have been prepared enzymatically. Mutants of Streptococcus lactis have been isolated by positive resistance and UV mutagenesis procedures. These mutants lack ATP-dependent glucokinase and/or PEP-mannose: phosphotransferase activities and are incapable of growth on glucose. Through the use of the bacterial mutants, and transport studies with (14C)-2F-lactose and (14C) 2D-lactose, definitive evidence for intracellular phosphorylation of free sugars via the PTS has been obtained. Biochemical, and physiological studies with Peptostreptococcus anaerobius have revealed that proline is a requirement for the transport and metabolism of glucose by this anaerobic oral pathogen.

膜调节的糖磷酸盐：磷酸水解酶已被纯化 并以干酪乳杆菌为特征。 该酶的抗体有 已获得用于结构/功能实验。 乳糖类似物 含有不可代谢的 (14C) 标记的 2-氟-D-葡萄糖或 2-脱氧-D-葡萄糖已通过酶法制备。 突变体 通过阳性耐药性和紫外线已分离出乳酸链球菌 诱变程序。 这些突变体缺乏 ATP 依赖性葡萄糖激酶 和/或 PEP-甘露糖：磷酸转移酶活性并且不能 葡萄糖生长。 通过使用细菌突变体和运输 (14C)-2F-乳糖和(14C)2D-乳糖的研究，明确的证据 游离糖通过 PTS 进行细胞内磷酸化 获得。 消化链球菌的生化和生理学研究 厌氧菌发现脯氨酸是运输的必需品 以及这种厌氧口腔病原体对葡萄糖的代谢。