REGULATION OF SUGAR TRANSPORT AND METABOLISM IN LACTIC ACID AND ORAL BACTERIA

乳酸和口腔细菌中糖运输和代谢的调节

• 批准号: 5201770
• 负责人: J THOMPSON
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF DENTAL & CRANIOFACIAL RESEARCH
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/5201770
• 关键词: 6 phosphofructokinase; Fusobacterium; Streptococcus lactis; aminoacid metabolism; bacterial genetics; carbohydrate metabolism; carbohydrate transport; chemical synthesis; cysteine; cytotoxicity; enzyme activity; gene expression; genetic regulation; lactates; lysine; molecular cloning; nucleic acid sequence; oral bacteria; ornithine; phosphotransferases; protein purification; protein sequence; site directed mutagenesis; triose phosphate isomerase

Microorganisms are causative or contributory agents in the etiology of dental caries, gingivitis and related periodontal disease(s). The pathogenicity of Gram-positive streptococci and Gram-negative Fusobacteria resides in part in the capacity of these species to form a variety of organic acids and toxic sulfur-containing derivatives as end products of carbohydrate and amino acid fermentation. Elucidation of the biochemical steps and the genetic basis for regulation of these energy-yielding pathways provides the rationale for this research program. Significant accomplishments of the past year include: 1) Demonstration that the triosephosphate gene (tpi) of Lactococcus lactis is monocistronic; 2) Elucidation of the role(s) of lysine 214 and cysteine residues in activity and cytotoxicity of beta-cystathionase from Bordetella avium; 3) Purification, cloning, sequence analysis and expression of maltose 6-phosphate:6-phosphohydrolase from Fusobacterium mortiferum ATCC 25557; 4) Cloning and site-directed mutagenesis of catalytically functional residues of Tn5306-encoded N(5)-(carboxyethyl) ornithine synthase from L. lactis; 5) Purification of a novel cellobiose 6-phosphate:6-phosphohydrolase from F. mortiferum; 6) Chemical synthesis of unique methylumbelliferyl analogs of phospho-alpha and phospho-beta-glucosides for use as fluorogenic reporter molecules for study of the regulation of gene expression in Fusobacteria. Results from this research program have been published in four papers in peer-reviewed, international journals.

微生物是疾病病因学中的致病因素或促成因素 龋齿、牙龈炎和相关牙周病。 这 革兰氏阳性链球菌和革兰氏阴性梭杆菌的致病性 部分取决于这些物种形成各种物种的能力 有机酸和有毒含硫衍生物作为最终产品 碳水化合物和氨基酸发酵。 生化的阐明 这些能量产生的调节步骤和遗传基础 路径提供了该研究计划的基本原理。 重要的 过去一年的成就包括： 1) 证明 乳酸乳球菌的磷酸三糖基因(tpi)是单顺反子； 2） 阐明 214 位赖氨酸和半胱氨酸残基在活性中的作用 以及来自鸟博德特氏菌的β-胱硫醚酶的细胞毒性； 3） 麦芽糖的纯化、克隆、序列分析和表达 6-磷酸：来自腐烂梭杆菌 ATCC 25557 的 6-磷酸水解酶； 4) 催化功能的克隆和定点突变 来自 L. 的 Tn5306 编码的 N(5)-(羧乙基)鸟氨酸合酶残基。 乳酸; 5) 新型纤维二糖的纯化 6-磷酸：来自 F. mortiferum 的 6-磷酸水解酶； 6) 化学合成 磷酸-α 的独特甲基伞形基类似物和 磷酸-β-葡萄糖苷用作荧光报告分子 梭杆菌基因表达调控的研究。 结果来自 该研究计划已在同行评审中发表了四篇论文， 国际期刊。