Light-guide Image Processing (LIP) 3D printed snapshot spectrometer for molecular imaging

用于分子成像的光导图像处理 (LIP) 3D 打印快照光谱仪

基本信息

批准号：
10447267
负责人：
TOMASZ S TKACZYK
金额：
$ 19.29万
依托单位：
RICE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2022
资助国家：
美国
起止时间：
2022-05-01 至 2024-02-29
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10447267
关键词：
3-Dimensional 3D Print Address Area Biological Biosensor Cancer Detection Cells Collection Color Complex Consumption Coupled Custom Cyclic AMP Data Development Devices Engineering Evaluation Event Face Fiber Fiber Optics Finland Fluorescence Resonance Energy Transfer Generations Geometry Germany Goals Image Imaging Device Imaging Techniques Investigation Islets of Langerhans Japan Light Light Microscope Lighting Measurement Methods Microscope Microscopy Morphologic artifacts Noise Optics Oranges Organizational Productivity Output Performance Photobleaching Phototoxicity Process Recording of previous events Research Research Personnel Resolution Rice Sampling Scanning Side Signal Transduction Spectrometry Speed Structure Structure of beta Cell of islet System Techniques Technology Testing Time Training Universities Washington Work base biological systems cellular imaging data acquisition experimental study fluorescence imaging fluorophore image guided image processing imaging modality imaging system improved innovative technologies insight instrument manufacturing process molecular imaging multidimensional data new technology photonics prototype quantum reconstruction remote sensing sensor spectrograph tool two-photon

项目摘要

Light-guide Image Processing (LIP) 3D printed snapshot spectrometer for molecular imaging The overall goal of this research is to develop a new generation of a high performance, compact, snapshot hyperspectral imaging device that is compatible with research grade light microscopes. To accomplish this goal we will build a Light-guide 3D printed Imaging Processing spectrometer. The LIP Spectrometer will leverage two innovative technologies: fiber optics and 2-photon additive manufacturing to allow effective fabrication of custom fiber bundles providing different input vs. output organizations. These custom bundles will capture densely packed input and yield an arbitrary output with void spaces that allow spectral information to be spread out. Thus, the LIP device will convert an image from 2D image (input) to separated spatial-spectral information (after dispersion) on a large format sCMOS/CCDs image sensor. The proposed approach requires no significant computation or processing to create the (x, y, λ) data cube. Simple data re-organization will be sufficient to create spectral cubes. LIP spectrometer will be a widefield method acquiring full spectral information simultaneously from every pixel and therefore it will offer significant advantages in imaging speed and signal collection. The LIP spectrometer will allow changing spectral / spatial sampling through different fiber bundle and disperser configurations. The project will pursue two specific aims: (1) developing a proof-of-concept LIP spectrometer (250x250x60 over visible range 470—670nm) and (2) testing the LIP Spectrometer the dynamic range, imaging speed and sampling over in reference to other techniques like IMS or scanning methods. We will also perform imaging in several living cell imaging applications. These experiments will focus on tests in dynamic biological systems, specifically to study [ATP]/[ADP] ratio, cAMP signaling, and Ca2+ dynamics investigated in Dr. Piston’s lab. We will also quantitatively evaluate the results from the LIP, against the Zeiss LSM880, Optical Insights Spectral DV commercial systems and Image Mapping Spectrometry (IMS) developed previously by project’s PI (Dr.Tkaczyk).

用于分子成像的光导图像处理 (LIP) 3D 打印快照光谱仪这项研究的总体目标是开发新一代高性能、紧凑、快照高光谱成像设备，与研究级光学显微镜兼容。为了实现这一目标，我们将建造一台光导 3D 打印成像处理光谱仪。 LIP 光谱仪将利用两项创新技术：光纤和 2 光子添加剂制造以允许有效制造定制光纤束，提供不同的输入与输出。这些自定义捆绑包将捕获密集的输入并产生一个输出组织。具有允许光谱信息展开的空白空间的任意输出，因此，LIP。设备会将图像从 2D 图像（输入）转换为分离的空间光谱信息（之后色散）在大格式 sCMOS/CCD 图像传感器上提出的方法不需要。创建 (x, y, λ) 数据立方体的大量计算或处理简单的数据重组。将足以创建光谱立方体。 LIP 光谱仪将是一种同时获取全光谱信息的宽场方法从每个像素，因此它将在成像速度和信号方面提供显着的优势 LIP 光谱仪将允许通过不同的方式改变光谱/空间采样。该项目将追求两个具体目标：（1）开发纤维束和分散器配置。概念验证 LIP 光谱仪（250x250x60，可见光范围 470—670nm）和 (2) 测试 LIP 光谱仪的动态范围、成像速度和采样优于其他光谱仪我们还将使用 IMS 或扫描方法等技术对多个活细胞进行成像。这些实验将集中于动态生物系统的测试，专门研究 [ATP]/[ADP] 比率、cAMP 信号传导和 Ca2+ 动力学。我们还将根据蔡司的 LIP 结果进行定量评估。 LSM880、Optical Insights Spectral DV 商业系统和图像映射光谱测量 (IMS) 之前由项目 PI (Tkaczyk 博士) 开发。