Modernization of 3-dimensional printing capabilities at the Aquatic Germplasm and Genetic Resource Center

水产种质和遗传资源中心 3 维打印能力的现代化

• 批准号: 10736961
• 负责人: Terrence Robert Tiersch
• 金额: $ 22.89万
• 依托单位: LOUISIANA STATE UNIV AGRICULTURAL CENTER
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-09-01 至 2024-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10736961
• 关键词: 3-Dimensional; 3D Print; Address; Adopted; Animals; Basic Science; Biomedical Research; Collaborations; Communities; Computers; Consumption; Cryopreservation; Custom; Defect; Development; Devices; Economics; Electrodes; Electronics; Engineering; Equipment; Faculty; Filament; Freezing; Funding; Genes; Genetic Transformation; Genetic study; Germ; Goals; Health; Human; Individual; Industrialization; Inferior; Infrastructure; Institution; Interdisciplinary Study; International; Investments; Knock-out; Laboratories; Maintenance; Metals; Microfabrication; Modeling; Modernization; Pathway interactions; Plant Resins; Postdoctoral Fellow; Prevention; Printing; Procedures; Process; Production; Protocols documentation; Quality Control; R24; Recovery; Reproducibility; Research; Research Personnel; Research Project Grants; Resource Sharing; Resources; Risk; Sampling; Science; Services; Site; Standardization; System; Technology; Time; Transgenic Organisms; United States Department of Agriculture; United States National Institutes of Health; Work; Zebrafish; agricultural center; aquatic organism; collaborative environment; commercialization; cost; genetic resource; graduate student; improved; innovative technologies; interdisciplinary approach; manufacturing technology; mechanical device; mutant; novel; open source; portability; preservation; prevent; programs; quality assurance; repository; research and development; screening; sensor; skills; technology development; tool; undergraduate student

PROJECT SUMMARY/ABSTRACT: Aquatic species have become accepted as valuable models in biomedical research, and laboratories around the world produce tens of thousands of mutant, knockout, gene-edited and transgenic lines each year. Establishment of efficient and reliable germplasm repositories is critical for preservation of genetic resources of aquatic organisms that are vital to advancing biomedical research. However, a pervasive lack of reproducibility in cryopreservation creates a significant barrier, posing a great risk of losing valuable lines developed from billions of dollars of research investment. There are two major problems: first, although protocols have been established through basic research, laboratories often produce low-quality samples because of a lack of affordable and reliable hardware for a standardized production pathway. Second, there are no effective approaches to provide quality management at a system level, including quality assurance for prevention of defects, and quality control for elimination of inferior products. Although some commercial options exist, most laboratories are not able to purchase expensive equipment when germplasm banking is not a focus or obligation. In previous R24 projects we worked with the Zebrafish International Resource Center (ZIRC) to establish strong on-site capabilities for them as a central facility. We also developed mechanical devices through 3-D printing and microfabrication to begin standardization efforts at ZIRC. We are expanding this technology initiative for widespread use across the steps required for comprehensive production and quality management pathways by development of multiple types of new scientific hardware that are practical, inexpensive, standardizable, portable, and customizable, and can be made available as user-driven public toolboxes through open hardware for thousands of laboratories. Over the past 10 years the AGGRC, a shared- use facility, has become a leader in production of open hardware for cryopreservation and repository development. To date, we have primarily restricted our work to consumer-level equipment to ensure that hardware distributed as printable computer files would be available to all users regardless of skill level. Although we are well-equipped for filament-based (e.g., plastic) 3-D printing, we have great need to substantially improve our ability to 3-D print with photocurable resins and metal. Modernized equipment is essential for a sustained powerful influence in this field. For example, we have adopted resin printing as an alternative to expensive and time-consuming microfabrication (e.g., photolithography), and require metal printing to work with resins to fabricate tools such as custom electrodes and sensors. Modernizing our capabilities will greatly streamline and improve operating processes and procedures, and it will provide much- needed access to technologically advanced equipment to support current, rapidly evolving, and emerging research programs. This will enable new and advanced approaches, offer innovative technological solutions, and benefit user communities and multiple research projects and investigators at our institution and beyond.

项目摘要/摘要： 水生物种已被接受为生物医学研究中的宝贵模型，而实验室周围已被接受 每年，世界都会产生成千上万的突变体，敲除，基因编辑和转基因线。 建立高效且可靠的种质存储库对于保存遗传资源至关重要 对于推进生物医学研究至关重要的水生生物。但是，普遍缺乏可重复性 在冷冻保存中，造成了重大障碍，构成了失去从 数十亿美元的研究投资。有两个主要问题：首先，尽管协议已经 通过基础研究建立，实验室经常生产低质量的样本，因为缺乏 标准化生产途径的负担得起且可靠的硬件。其次，没有效果 在系统级别提供质量管理的方法，包括预防质量保证 缺陷和消除劣质产品的质量控制。尽管存在一些商业选择，但大多数 当种质银行不是重点或 义务。在以前的R24项目中，我们与斑马鱼国际资源中心（ZIRC）合作 为他们作为中央设施建立强大的现场功能。我们还开发了机械设备 通过3-D打印和微加工开始在Zirc上开始标准化工作。我们正在扩大这个 在全面生产和质量所需的步骤中广泛使用的技术计划 通过开发多种类型的新科学硬件的管理途径，这些硬件是实用的， 便宜，可标准化，便携式和可定制，可以作为用户驱动的公共 通过开放硬件的工具箱，用于成千上万的实验室。在过去的10年中，Aggrc，共享 - 使用设施，已成为生产开放硬件的领导者，用于冷冻保存和存储库 发展。迄今为止，我们主要将工作限制在消费者级设备上，以确保 不管技能水平如何，所有用户都可以使用作为可打印计算机文件分布的硬件。 尽管我们配备了基于细丝的（例如塑料）3-D打印的功能良好，但我们非常需要 通过可光化的树脂和金属，实质上提高了我们3-D打印的能力。现代化设备是 对于在这一领域持续强大的影响力至关重要。例如，我们采用了树脂印刷 替代昂贵且耗时的微加工（例如，光刻），需要金属 打印以与树脂一起制造工具，例如定制的电极和传感器。现代化我们的 功能将极大地简化和改善操作流程和程序，它将提供很多 需要访问技术先进的设备，以支持当前，快速发展和新兴 研究计划。这将实现新的和高级的方法，提供创新的技术解决方案， 并使我们机构及其他地区的用户社区和多个研究项目和研究人员受益。