ENHANCED DEVELOPMENT OF THE XIPHOPHORUS MODEL SYSTEM

XIPHOPHORUS 模型系统的增强开发

• 批准号: 8145809
• 负责人: Terrence Robert Tiersch
• 金额: $ 61.16万
• 依托单位: TEXAS STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-04-01 至 2016-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8145809
• 关键词: Alleles; Animal Experimentation; Animal Model; Animals; Award; Backcrossings; Behavior; Belize; Betula Genus; Biological Models; Biology; Biomedical Research; Book Chapters; Breeding; Canada; China; Code; Collection; Communities; Complex; Consultations; Country; Cryopreservation; Custom; Data; Databases; Denmark; Development; Disease; Dissection; Embryo; England; Environment; Experimental Models; Female; Fishes; Fundulus; Gene Expression; Gene Expression Profile; Genes; Genetic; Genetic Markers; Genome; Genotype; Germ Cells; Germany; Glean; Goals; Guatemala; Habitats; Housing; Human; Hybrids; Inbreeding; Individual; Inheritance Patterns; Institution; Israel; Japan; Knowledge; Laboratories; Lead; Life; Link; Manuals; Mechanics; Methodology; Methods; Mexico; Modeling; Molecular; Monoclonal Antibody R24; Monograph; National Center for Research Resources; Neoplasms; Oviparity; Paper; Parents; Partner in relationship; Pattern; Phenotype; Population; Predisposition; Principal Investigator; Process; Production; Protocols documentation; Publications; Publishing; Regulator Genes; Relative (related person); Repetitive Sequence; Research; Research Personnel; Resources; Role; Science; Scientist; Shipping; Ships; Singapore; Site; Staging; Taxon; Texas; United States; Universities; Variant; Vertebrates; Xiphophorus; Zebrafish; abstracting; animal resource; base; catalyst; cohort; college; design; egg; functional genomics; gene interaction; genetic strain; interest; male; meetings; melanoma; next generation; programs; repository; research study; sperm cell; symposium; trait; tumor; tumorigenesis

DESCRIPTION (provided by applicant): Xiphophorus fishes have been used as a vertebrate experimental model for fundamental biomedical research for over 60 years. This R24 proposal outlines both resource and research components designed to maintain and enhance the Xiphophorus Genetic Stock Center (XGSC) that has operated within the United States since the 1930's making it one of the oldest live animal resources centers worldwide and a national scientific treasure. The XGSC houses over 60 pedigreed genetic lines representing 25 Xiphophorus species in over 1,400 aquaria. The XGSC has provided pedigreed fish and materials to researchers in over 30 laboratories representing 11 countries. The traditional strength of the Xiphophorus experimental model involves the non-biased assessment of genetic inheritance patterns associated with complex phenotypes within intact animals. For example, genetic control of tumor susceptibility has been documented in both pure Xiphophorus strains and select interspecies hybrids, for a variety of spontaneous and induced neoplasms including several genetically distinct melanomas. Our preliminary studies represent a first global comparison of the relative abundance of species-specific allele expression in two vertebrate species and Fl hybrids. In this initial study we were able to identify substantial changes in gene expression levels for parental alleles in the interspecies hybrid genetic background. We also found a new class of genetic interactions that lead to substantially suppressed gene expression in Fl hybrids relative to either parent. These initial experiments, employing RNAseq to follow allele specific gene expression in Fl interspecies hybrids, serve as examples of the Xiphophorus model may be used to uncover new knowledge regarding the mechanics of gene interactions, the genetic basis of developmental programs, complex behaviors, and the molecular processes underlying disease initiation and progression. If the goals of this proposal are realized, mechanistic dissection of Xiphophorus interspecies hybrid melanoma models may be approached and the results gleaned will have translational significance to tumorigenesis in humans. PUBLIC HEALTH RELEVANCE (provided by applicant): If the goals of this proposal are realized, mechanistic dissection of Xiphophorus interspecies hybrid melanoma models may be approached since genome and transcriptome sequences of both parental species will allow assessment of genetic regulatory changes that produce an individual that is predisposed to melanoma. These results will have direct translational significance to tumorigenesis in humans.

描述（由申请人提供）：Xiphophorus鱼类已被用作基本生物医学研究的脊椎动物实验模型已有60多年了。该R24提案概述了旨在维护和增强Xiphophorus遗传储备中心（XGSC）的资源和研究组件，该中心自1930年代以来一直在美国境内运作，这使其成为全球最古老的活动物资源中心之一和国家科学宝藏。 XGSC在1,400多个水族箱中占有60多种典型的遗传线，代表25种Xiphophorus物种。 XGSC已向代表11个国家 /地区的30多个实验室的研究人员提供了建鱼和材料。 Xiphophorus实验模型的传统强度涉及对与完整动物中复杂表型相关的遗传遗传模式的无偏见评估。例如，在纯Xiphophorus菌株和选择种间杂种中，遗传控制均已记录在各种自发和诱导的肿瘤中，包括几种遗传上不同的黑色素瘤。 我们的初步研究是对两个脊椎动物和FL杂种中物种特异性等位基因表达相对丰度的首次全球比较。在这项最初的研究中，我们能够确定种间杂交遗传背景中父母等位基因的基因表达水平的实质变化。我们还发现了一类新的遗传相互作用，导致相对于任何一个父母，在FL杂种中大大抑制了基因表达。这些最初的实验采用RNASEQ遵循等位基因的基因表达在FL种间杂种中作为Xiphophorus模型的例子，可用于发现有关基因相互作用机制的新知识，发展程序的遗传基础，复杂行为和复杂的行为以及疾病疾病启动的分子过程。如果实现了该提案的目标，则可以实现Xiphophorus种间杂交黑色素瘤模型的机械解剖，并且收集的结果将对人类的肿瘤发生具有翻译意义。 公共卫生相关性（由申请人提供）：如果实现了该提案的目标，则可以接近Xiphophorus种间杂交黑色素瘤模型的机械解剖，因为这两个亲本物种的基因组和转录组序列将允许评估遗传调节性变化，从而产生一种对黑素瘤倾向的人。这些结果将对人类的肿瘤发生具有直接的翻译意义。

项目成果

Proteomic analyses of the Xiphophorus Gordon-Kosswig melanoma model.

剑尾鱼 Gordon-Kosswig 黑色素瘤模型的蛋白质组学分析。

• DOI: 10.1016/j.cbpc.2011.05.017
• 发表时间: 2012
• 期刊: Comparative biochemistry and physiology. Toxicology & pharmacology : CBP
• 作者: Perez,AmyN;Oehlers,Lee;Heater,SheliaJ;Booth,RachellE;Walter,RonaldB;David,WendiM
• 通讯作者: David,WendiM

Identification of transcriptome SNPs between Xiphophorus lines and species for assessing allele specific gene expression within F₁ interspecies hybrids.

• DOI: 10.1016/j.cbpc.2011.03.012
• 发表时间: 2012-01
• 期刊: COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
• 影响因子: 3.9
• 作者: Shen, Yingjia;Catchen, Julian;Garcia, Tzintzuni;Amores, Angel;Beldorth, Ion;Wagner, Jonathan;Zhang, Ziping;Postlethwait, John;Warren, Wes;Schartl, Manfred;Walter, Ronald B.
• 通讯作者: Walter, Ronald B.

RNA-Seq reveals complex genetic response to Deepwater Horizon oil release in Fundulus grandis.

• DOI: 10.1186/1471-2164-13-474
• 发表时间: 2012-09-12
• 期刊: BMC genomics
• 影响因子: 4.4
• 作者: Garcia TI;Shen Y;Crawford D;Oleksiak MF;Whitehead A;Walter RB
• 通讯作者: Walter RB

Silver nanospheres are cytotoxic and genotoxic to fish cells.

• DOI: 10.1016/j.aquatox.2009.11.016
• 发表时间: 2010-04-01
• 期刊: Aquatic toxicology (Amsterdam, Netherlands)
• 作者: Wise JP Sr;Goodale BC;Wise SS;Craig GA;Pongan AF;Walter RB;Thompson WD;Ng AK;Aboueissa AM;Mitani H;Spalding MJ;Mason MD
• 通讯作者: Mason MD

Transcriptome analysis of female and male Xiphophorus maculatus Jp 163 A.

• DOI: 10.1371/journal.pone.0018379
• 发表时间: 2011-04-05
• 期刊: PloS one
• 影响因子: 3.7
• 作者: Zhang Z;Wang Y;Wang S;Liu J;Warren W;Mitreva M;Walter RB
• 通讯作者: Walter RB