Anthropo-genetics on paleomongoloid in South America

南美洲古蒙古人种的人类遗传学

• 批准号: 09041195
• 负责人: TAJIMA Kazuo
• 金额: $ 5.7万
• 依托单位: Aichi Cancer Center
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for international Scientific Research
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09041195/
• 关键词: Japanese; Andeans; Mummy; DNA; HTLV human; beta-globin; Mitochondrial DNA; Ethnoepidemiology; HLA

To c1arify the paleoanthropological relationship between Japanese and Andeans, characteristics of provirus DNA of human T-cell leukemia virus (HTLV), mitochondrial DNA and nuclear DNA were compared among materials : 1) high and low landers in South America ; 2) Andean mummies ; and 3) southern Japanese. From DNA analysis of HTLV and human leukocyte antigens, it was clarified that high landers in the Andes area were closely related to southern Japanese, but people in other low lands were exclusively different from them. Specific gene human beta-globin was identified from two out of 104 mummies collected in Andes areas. Furthermore, one out two beta-globin positive Andean mummies about 1,500 years ago was positive for HTLV-I pX, too. Base analysis of D-loop regeon of mitochondrial DNA from 90 mummies in north Chile were completed and they showed four specific groups of mitochondrial DNA asshown in contemporary people in South America. These results porvides the first evidence that HTLV-I was carried by ancient Mongoloids to the Andes before the Colonial era. .

为了阐明日本人和安第斯人之间的古人类学关系，我们比较了以下材料的人类T细胞白血病病毒（HTLV）原病毒DNA、线粒体DNA和核DNA的特征：1）南美洲的高地和低地登陆者； 2）安第斯木乃伊； 3）日本南部​​。从HTLV和人类白细胞抗原的DNA分析中可以看出，安第斯山脉地区的高地人与日本南部的人有密切的亲缘关系，但其他低地地区的人与他们完全不同。从安第斯山脉地区收集的 104 具木乃伊中的两具中鉴定出了特定的人类 β-珠蛋白基因。此外，大约 1,500 年前的安第斯山木乃伊中，两具 β 珠蛋白呈阳性的木乃伊中，就有两具 HTLV-I pX 呈阳性。对智利北部 90 具木乃伊线粒体 DNA D 环区域的基础分析已完成，结果显示南美洲当代人具有四种特定的线粒体 DNA 组。这些结果提供了第一个证据，证明HTLV-I是在殖民时代之前由古代蒙古人携带到安第斯山脉的。 。

项目成果

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Blagitko, N. 等人：“哥伦比亚、厄瓜多尔和智利美洲人中 HLA-DRB1 位点的多形性”人类免疫学 (Human Immunol.)54(1)。

Nishio, I.et al.: "A new congenital muscular dystrophy with mitochondrial structural abnormalities." Muscle & Nerve. 21. 40-47 (1998)

Nishio, I.et al.：“一种新的先天性肌营养不良症，伴有线粒体结构异常。”

Takamoto, T.: "HTLV-linfected T cells activate autologous CD4^+ T cells susciptible to HILV-I injection in a costimulatory molecule-dependent fashion" Eur.J.Immunol.27. 1427-1432 (1997)

Takamoto, T.：“HTLV 感染的 T 细胞以共刺激分子依赖的方式激活对 HILV-I 注射敏感的自体 CD4+ T 细胞”Eur.J.Immunol.27。

Ozawa, M.et al.: "Myoclonus epilepsy associated with ragged-red fibers : A G-to-A mutation at nucleotide pair 8363 in mitochondrial tRNA^<Lys> in to families." Muscle & Nerve. 20. 271-278 (1997)

Ozawa, M.et al.：“与参差不齐的红色纤维相关的肌阵挛癫痫：家族中线粒体 tRNA^<Lys> 核苷酸对 8363 处的 G-to-A 突变。”

Miura, T.et al.: "Molecular phylogency of human T-cell leukemia virus type I'and II of Amerindians in Colonbia and Chile." J.Mol.Evol.44. S76-S82 (1997)

Miura, T.等人：“哥伦比亚和智利美洲印第安人 I 型和 II 型人类 T 细胞白血病病毒的分子系统发育。”