Study on Higher Order Phase Transition of Phospholipid Bilayrs and Stability of Emulsion Formed with These Disperisons

磷脂双层的高阶相变及其分散体乳液的稳定性研究

• 批准号: 06453059
• 负责人: TAJIMA Kazuo
• 金额: $ 4.74万
• 依托单位: Kanagawa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-06453059/
• 关键词: ESR spectra for phospholipid bilayr; Thermal properties of DMPC; Thermal properties of DMPG; Higher order phase transition in bilayr; Emulsifier of phospholipid; Stability of emulsion; Coalescene kinetics; Preparation of emulsion with phospholipids

This work was carried out to make clear the thermal properties of lamellar liquid crystals of dimyristoylphosphatidylcholine (DMPC) and dimyristoryphosphatidylglycerol (DMPG) dispersed in water, and emulsions of hexadecane formed with these lipids. Main measurements for the lipid dispersions were made on the temperature dependence of heat capacity at constant pressure, equilibrium apread pressure on water, X-ray diffraction analysis, ESR spectra, DSC,and zeta-Potential.As a result, it was confirmed from these experiments that in the lamellar liquid crystals of the (L_<alpha>+H_2O) phase a higher order phase transition of bilayr occurred at the temperatures several degrees higher than their gel-liquid crystral transition temperatures (Tm ; 23.5ﾟC for DMPC and 23.7ﾟC for DMPG). The higher order phase transition for these lipids were observed at 29.0ﾟC for DMPC dispersion and at 31.7ﾟC for DMPG dispersion. The physical and surface-chemical properties of these phospholipids were found to d … More iffer incredibly below and above T^<**>.Subsequently, the emulsification of hexadecane with DMPC dispersion was investigated below and above T^<**> temperature. It was found that the particle size, zeta-potential, and thermal stability of emulsion were changed in paralell with the variation of the thermal properties of DMPC dispersion at T^<**>. Especially, a new finding was that when hexadecane wa emulsified with DMPC at a temperature between Tm and T^<**>, the emulsion surface was covered and stabilized with the triple layrs of DMPC,but above T^<**> the surface of emulsion was composed of a monolayr of DMPC as a usual surfactant. This observation was supported by the precise study on the coalescence kinetics for the DMPC stabilized hexadecane emulsion. The conclusion was that the activation energy for coalescence of the emulsion taking place below T^<**> was twice lager than that for coalescence above T^<**>. This may explain why the emulsion formed with DMPC above T^<**> became less stable. Less

进行这项工作是为了清除二甲酰磷脂酰胆碱（DMPC）（DMPC）和Dimyristory磷脂酰甘油（DMPG）的层状液体晶体的热性能，并在水中散发出来，以及由这些脂质形成的六烷烷的乳液。对脂质分散体的主要测量是根据恒定压力，水的平衡压力，X射线衍射分析，ESR光谱，DSC和Zeta-potential进行的主要测量，从这些实验中证实，在这些实验中证实，在lamellar液晶中，在lamellar液晶中会发生较高的（L_ <Alpha>+H_2O）的阶段。比其凝胶液晶体过渡温度高几个度（TM； DMPC的23.5°C，DMPG的23.7°C）。 DMPC分散剂在29.0°C下观察到这些脂质的高阶跃迁，DMPG分散剂的高阶转变。发现这些磷脂的物理和表面化学特性在t^<**>上令人难以置信地令人难以置信，随后，在T^<**>以上，研究了用DMPC分散剂的六二烷化的溶液化。已经发现，随着t^<**>时DMPC分散体的热性能的变化，乳液的粒度，ZETA功能和乳液的热稳定性发生了变化。尤其是一个新的发现是，当六烷基WA用DMPC在TM和T^<**>之间的温度下乳化时，乳液表面被覆盖并用DMPC的三重层盖住并稳定在T^<**>上面，但乳液表面由DMPC的A Monolayr组成。该观察结果得到了关于DMPC稳定六烷乳液的合并动力学的精确研究。这可以解释为什么在T^<**>上形成的DMPC形成的乳液变得不那么稳定。较少的

项目成果

K.Tajima,Y.Imai,et al: "ESR Study on DMPC and DMPG Bilayers in the (L_a+H_2O) Phase" Langmiur. 12. 6651-8 (1996)

K.Tajima、Y.Imai 等人：“(L_a H_2O) 相中 DMPC 和 DMPG 双层的 ESR 研究”Langmiur。

K.Tajima, Y.Imai, K.Ando, A.Nakamura, M.Koshinuma: "X-ray diffraction Study on Smectic Liquid Crystal State of Dimyristoylphosphatidylcholine" Chemistry Lettes. 1995. 527 (1995)

K.Tajima、Y.Imai、K.Ando、A.Nakamura、M.Koshinuma：“二肉豆蔻酰磷脂酰胆碱近晶液晶态的 X 射线衍射研究”化学快报。

K.Tajima, Y.Imai, M.Koshinuma, A.Nakamura: "Thermal Properties of Hexadcane Emulsion Stabilized with Dimyristoylphosphatidylcholine" J.Jpn.Oil Chem.Soc.46 (3). 283-291 (1997)

K.Tajima、Y.Imai、M.Koshinuma、A.Nakamura：“用二肉豆蔻酰磷脂酰胆碱稳定的十六烷乳液的热性能”J.Jpn.Oil Chem.Soc.46 (3)。

K.Tajima,Y.Imai,et al: "Coalescence Kinetics of Hexadecane Emulsion Formed with Dimyristoylphosphatidylchiline" J.Jpn.Oil Chem.Soc.46. (1997)

K.Tajima、Y.Imai 等人：“用二肉豆蔻酰磷脂酰基啉形成的十六烷乳液的聚结动力学”J.Jpn.Oil Chem.Soc.46。

K.Tajima,Y.Imai,et al: "Thermal Properties of Hexadecane Emulsion Stabilized with Dimyristoylphosphatidylcholine" J.Jpn.Oil Chem.Soc.46. 283-291 (1997)

K.Tajima、Y.Imai 等人：“用二肉豆蔻酰磷脂酰胆碱稳定的十六烷乳液的热性能”J.Jpn.Oil Chem.Soc.46。