Relationships between gene diversity and organismal evolution

基因多样性与生物进化的关系

• 批准号: 09304072
• 负责人: MIYATA Takashi
• 金额: $ 22.98万
• 依托单位: KYOTO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09304072/
• 关键词: Sponge; Cyclostomes; Gene family; Gene duplication; Phylogenetic tree; Organismal diversity; 生物多様性; シグナル伝達系; 形態形成遺伝子; 多様性; 真核生物; 多細胞効物; 進化

Animals evolved a variety of gene families involved in cell-cell communication and developmental control by gene duplication and domain shuffling. Each family comprises several subtypes or subfamilies with distinct structures and functions which diverged by gene duplications and domain shuffling before the divergence of parazoans and eumetazoans. Since the separation from protostomes, vertebrates expanded the multiplicity of members (isoforms) in the same subfamily by further gene duplications in their early evolution before the fish-tetrapod split. To know the dates of isoform duplications more closely, we have conducted isolation and sequencing of genes belonging to four different subtypes of the protein tyrosine kinase family, five subtypes of protein tyrosine phosphatase family, and other three gene families from Branchiostoma belcheri, an amphioxus, Eptatretus burgeri, a hagfsh and Lampetra reissneri, a lamprey. From a phylogenetic tree of each subfamily inferred from a maximum likelihood (ML) method, together with a bootstrap analysis based on the ML method, we have shown that the isoform duplications frequently occurred in the early evolution of vertebrates around or just before the divergence of cyclostomes and gnathostomes by gene dupli-cations and possibly chromosomal duplications.

动物通过基因复制和域进行改组，进化了各种参与细胞 - 细胞通信和发育控制的基因家族。每个家庭都包含几种亚型或亚科，具有不同的结构和功能，这些结构和功能因基因的复制而差异，并且在帕拉索人和eumetazoans发散之前进行了差异。由于与原生体的分离，脊椎动物通过在鱼 - 四足动物分裂之前的早期进化中进一步的基因重复，在同一亚科中扩大了成员（同工型）的多样性。为了更紧密地了解同工型重复的日期，我们已经对属于蛋白质酪氨酸激酶家族的四种不同亚型的基因进行了隔离和测序七lamp。从根据ML方法的最大可能性（ML）方法推断的每个亚家族的系统发育树，以及基于ML方法的自举分析，我们表明，同工型重复经常发生在脊椎动物的早期演变中，或者在cyclostomes and Gnathostomes和Gnathostomes之前的基因和gnathostomes之前的基因和可能的dupli-dupli-duers和可能的chromecom和可能的chrols-可能的chrols可能。

项目成果

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须贺，H.

Suga, H. et al.: "Protein tyrosine kinase cDNAs from amphioxus, hagfish and lamprey : lsoform duplications around the divergence of cyclostomes and gnathostomes."J. Mol. Evol. 49. 601-608 (1999)

Suga, H. 等人：“来自文昌鱼、盲鳗和七鳃鳗的蛋白质酪氨酸激酶 cDNA：环口目和颌口目分歧周围的同形重复。”J.

Matsuda,F. et al,: "The complete nucleotide sequence of the human immunoglobulin heavy-chain varoab;e region locus"J. Exp. Med.. 188. 2151-2162 (1998)

松田，F.

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Katoh K.等人：“用于推断系统发育树的最大似然法的启发式方法以及对睾丸决定基因 SRY 的哺乳动物 SOX-3 起源的应用”FEBS Lett.. 463. 129-132 (1999)