Relationships between gene diversity and organismal evolution

基因多样性与生物进化的关系

• 批准号: 09304072
• 负责人: MIYATA Takashi
• 金额: $ 22.98万
• 依托单位: KYOTO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09304072/
• 关键词: Sponge; Cyclostomes; Gene family; Gene duplication; Phylogenetic tree; Organismal diversity; 生物多様性; シグナル伝達系; 形態形成遺伝子; 多様性; 真核生物; 多細胞効物; 進化

Animals evolved a variety of gene families involved in cell-cell communication and developmental control by gene duplication and domain shuffling. Each family comprises several subtypes or subfamilies with distinct structures and functions which diverged by gene duplications and domain shuffling before the divergence of parazoans and eumetazoans. Since the separation from protostomes, vertebrates expanded the multiplicity of members (isoforms) in the same subfamily by further gene duplications in their early evolution before the fish-tetrapod split. To know the dates of isoform duplications more closely, we have conducted isolation and sequencing of genes belonging to four different subtypes of the protein tyrosine kinase family, five subtypes of protein tyrosine phosphatase family, and other three gene families from Branchiostoma belcheri, an amphioxus, Eptatretus burgeri, a hagfsh and Lampetra reissneri, a lamprey. From a phylogenetic tree of each subfamily inferred from a maximum likelihood (ML) method, together with a bootstrap analysis based on the ML method, we have shown that the isoform duplications frequently occurred in the early evolution of vertebrates around or just before the divergence of cyclostomes and gnathostomes by gene dupli-cations and possibly chromosomal duplications.

动物通过基因复制和结构域改组进化出多种参与细胞间通讯和发育控制的基因家族。每个科都包含几个具有不同结构和功能的亚型或亚科，在副动物和真后动物分化之前，这些亚型或亚科因基因复制和结构域改组而分化。自从与原口动物分离以来，脊椎动物在鱼类四足动物分裂之前的早期进化中通过进一步的基因复制扩大了同一亚科成员（亚型）的多样性。为了更准确地了解异构体重复的日期，我们对属于蛋白酪氨酸激酶家族的四种不同亚型、蛋白酪氨酸磷酸酶家族的五种亚型以及来自文昌鱼的其他三个基因家族的基因进行了分离和测序。 Eptatretus Burgeri（一种七鳃鳗）和 Lampetra reissneri（一种七鳃鳗）。根据最大似然（ML）方法推断出的每个亚科的系统发育树，以及基于ML方法的引导分析，我们发现，亚型重复经常发生在脊椎动物的早期进化中，大约或之前的分歧。圆口动物和颚口动物通过基因复制和可能的染色体复制。

项目成果

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Matsuda, F. 等人：“人免疫球蛋白重链可变区基因座的完整核苷酸序列。”J.

Suga,H. et al.: "Multiple TGF-b receptor related genes in sponge and ancient gene duplications before the parazoan-eumetazan split"FEBS Lett.. 453. 346-350 (1999)

须贺，H.

Suga, H. et al.: "Protein tyrosine kinase cDNAs from amphioxus, hagfish and lamprey : lsoform duplications around the divergence of cyclostomes and gnathostomes."J. Mol. Evol. 49. 601-608 (1999)

Suga, H. 等人：“来自文昌鱼、盲鳗和七鳃鳗的蛋白质酪氨酸激酶 cDNA：环口目和颌口目分歧周围的同形重复。”J.

Katoh K.et al.: "A heuristic approach of maximum likelihood methood for inferring phylogenetic tree and an application to the mammalian SOX-3 origin of the testis-determining gene SRY"FEBS Lett.. 463. 129-132 (1999)

Katoh K.等人：“用于推断系统发育树的最大似然法的启发式方法以及对睾丸决定基因 SRY 的哺乳动物 SOX-3 起源的应用”FEBS Lett.. 463. 129-132 (1999)

Suga,H. et al.: "Extensive gene duplication in the early evolution fo animals befor parazoan-eumetazoan split demonstrated by G proteins and protein tyrosine kinases from sponge and hydra"J. Mol. Evol.. 48. 646-653 (1999)

须贺，H.