Synthesis of Biohybrid Gels That Respond to Signal Biomolecules and Their Biomedical Applications

响应信号生物分子的生物混合凝胶的合成及其生物医学应用

基本信息

批准号：
15300173
负责人：
MIYATA Takashi
金额：
$ 10.56万
依托单位：
Kansai University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2005
项目状态：
已结题

项目摘要

In this study, we tried to prepare (a) biomolecule-crosslinked gels and (b) biomolecule-imprinted gels as biohybrid gels that undergo volume changes in response to signal biomolecules :(a) Biomolecule-crosslinked gelsReversibly glucose-responsive gels were prepared by using complex formation between lectins and polymers with pendant glucose. Biohybrid gels having antigen-antibody complexes as crosslinking points swelled immediately in the presence of a target antigen and shrank again in its absence. The antigen-responsive gels controlled the drug permeation in response to the antigen concentration. DNA-responsive gels were prepared by introducing duplex DNA at crosslinking points. Swelling behavior of the duplex DNA-crosslinked gels was strongly dependent upon the sequence of DNA. Measurements of crosslinking density of their biohybrid gels revealed that their biomolecule-responsive swelling is due to the dissociation of the biomolecular complexes in the presence of a target biomolecul … More e.(b) Biomolecule-imprinted gelsGlycoprotein-imprinted gels were prepared by biomolecular imprinting in which lectin and antibody were utilized as ligands for a target tumor-marker glycoprotein. The glycoprotein-imprinted gels only shrank when both lectin and antibody in the gels simultaneously recognized the saccharide and peptide chains of the target glycoprotein. The glycoprotein-responsive shrinking of the imprinted gel was due to formation of lectin-glycoprotein-antibody complexes that acted as reversible crosslinking points. Furthermore, DNA-imprinted gels having two ligand DNAs shrank in a buffer solution containing a target DNA but swelled a little in the presence of DNAs with different sequences. The responsive shrinking behavior of the bimolecule-imprinted gels is attributed to an increase in their crosslinking density by complex formation between ligands and target biomolecules.As biomolecule-responsive behavior of biomolecule-crosslinked gels and biomolecule-imprinted gels enables the accurate detection and recognition of target biomolecules, they have many potential applications as smart devices in sensing systems and as molecular diagnostics. Less

在本研究中，我们尝试制备（a）生物分子交联凝胶和（b）生物分子印迹凝胶作为生物混合凝胶，其体积随信号生物分子而变化：（a）生物分子交联凝胶可逆葡萄糖响应凝胶的制备方法使用凝集素和带有悬垂葡萄糖的聚合物之间的复合物形成，具有抗原-抗体复合物作为交联点，在凝胶中立即膨胀。通过在交联点引入双链体 DNA，抗原反应性凝胶可根据抗原浓度控制药物渗透。凝胶强烈依赖于 DNA 序列。对其生物混合凝胶的交联密度的测量表明，其生物分子响应性膨胀是由于 DNA 的解离造成的。 e.(b) 生物分子印迹凝胶糖蛋白印迹凝胶是通过生物分子印迹制备的，其中凝集素和抗体用作目标肿瘤标志物糖蛋白的配体。仅当凝胶中的凝集素和抗体同时识别目标糖蛋白的糖链和肽链时，凝胶才会收缩。印迹凝胶的糖蛋白响应性收缩是由于凝集素-糖蛋白-抗体复合物的形成，该复合物充当可逆交联点。此外，具有两个配体DNA的DNA印迹凝胶在含有靶DNA的缓冲溶液中收缩，但在含有目标DNA的缓冲溶液中稍微膨胀。双分子印迹凝胶的响应收缩行为归因于配体与DNA之间形成复合物而增加了其交联密度。由于生物分子交联凝胶和生物分子印迹凝胶的生物分子响应行为能够准确检测和识别目标生物分子，因此它们作为传感系统中的智能设备和分子诊断具有许多潜在应用。