The effects of three-dimensional form on the healing process of three-dimensional alveolar bone defect.

三维形态对三维牙槽骨缺损愈合过程的影响。

基本信息

批准号：
11672087
负责人：
MIYATA Takashi
金额：
$ 1.15万
依托单位：
Meikai University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2001
项目状态：
已结题

项目摘要

The Purpose of this study was to clarify the healing process and establish the standard of the effect of periodontal tissue re-generation ability. The experimental animals for make 3-wall bone defects were six male crab-eating monkeys (Macaca fascicularis), each monkeys were approximately 2 to 3 years of age and weighting 3.8 to 5.7 kg. The monkeys were kept in separate metal cages under constant temperature and humidistat conditions (28 ± 1℃, 50% to 60%) with sufficient solid monkey dietand tap of water. The oral hygiene procedures were performed once a week under general anesthesia to maintain the periodontal tissues in as healthy condition as possible. The test sites were the second premolar and first molar teeth in the right and left mandible.The second premolar and the first molar in the right and left mandible were extracted under local anesthesia with 2% lidocine, including epinephrine for hemostasis. Three months after, full-thickness mucoperiosteai flap with vertical releaseing incisions were raised, and made various size of artificial bone defect surrounding teeth.Control group were scaled the surface of the root and close the flap. Boneject group were implant artificial bone material (Boneject^【encircled R】, Koken Co.Ltd., Japan) and close the flap. Membrane group were used resorbable membrane (Resolut^【encircled R】, Japan Gore-tex, Japan). Three month after surgery, animals were sacrificed and made tissue section. In the results, 2x1x1 bone defect were fully filled by bone tissue in all groups. In contrast, 2x2x2 or 2x2x4 bone defects, they were no bony healing in the control group. In these results suggest that, they are no effect on the small bony defect but more than 8mm^3 of bony defect, some regenerative therapy will be usefull to regenerate periodontal tissue.

这项研究的目的是阐明愈合过程并确定牙周组织再产生能力的影响的标准。用于三壁骨缺损的实验动物是六只雄性螃蟹猴（Macaca fascicularis），每个猴子大约2至3岁，体重为3.8至5.7 kg。在恒定温度下，将猴子保存在单独的金属笼子中，并加湿条件（28±1℃，50％至60％），并有足够的固体猴子饮食和水水龙头。口服卫生程序每周在全身麻醉下进行一次，以使牙周组织保持尽可能健康的状态。测试地点是左右下颌骨的第二前磨牙和第一摩尔牙齿。在局部麻醉下提取第二次和左下颌骨的第二磨牙和第一磨牙，其中包括2％Lidocine，包括淋巴结肾上腺素。三个月后，抬高了垂直释放切口的全厚性粘膜粘膜皮瓣，并使牙齿周围的各种人造骨缺损。对照组缩放了根部的表面并关闭皮瓣。骨骼组是植入人造骨料（骨头^【环绕的r】，koken co.ltd。，日本），并关闭皮瓣。使用膜组吸收膜（Japan Gore-Tex，Japan，Japan）。手术后三个月，牺牲动物并制作组织部分。在结果中，所有组中的骨组织完全填充了2x1x1骨缺损。相反，2x2x2或2x2x4骨缺损，它们在对照组中没有粘结愈合。在这些结果表明，它们对小粘结缺陷没有影响，但粘结缺陷超过8mm^3，某些再生治疗将对再生牙周组织有用。