Phylogenetic aspects of the cyclostomes with special attention to the primitive complement sysytem.

环口动物的系统发育方面，特别关注原始补体系统。

• 批准号: 13640700
• 负责人: FUJII Tamotsu
• 金额: $ 2.24万
• 依托单位: Hiroshima Prefectural Women's University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13640700/
• 关键词: Complement; Innate immunity; Lectin pathway; Cyclostome; Hagfish; C3 convertase; MASP; Serine protease; アナフィラトキシン; 補体第3成分

Hagfish is the most, primitive extant vertebrate and thought to defend itself against pathogens only by innate immune system. With a view to understanding the evolution of the innate immune system, we have isolated complement C3 from the serum of hagfish. In this study, our attempt to identify a hagfish C3 convertase was carried out by RT-PCR using degenerative primers designed on the basis of the conserved amino acid stretches among the several kinds of serine proteases. Analyses of a full-length cDNA clone isolated from a hagfish liver cDNA library using one of the cloned RT-PCR products as a probe indicated that this cDNA encoded hagfish mannose-binding lectin-associated serine protease WASP)1. This evidence strongly suggests that the hagfish defends itself against pathogens at least by the complement system composed of lectin pathway

盲鳗是现存最原始的脊椎动物，人们认为只能通过先天免疫系统来防御病原体。为了了解先天免疫系统的进化，我们从盲鳗的血清中分离出了补体C3。在本研究中，我们尝试通过 RT-PCR 进行鉴定盲鳗 C3 转化酶，使用根据几种丝氨酸蛋白酶之间的保守氨基酸序列设计的简并引物。使用克隆的 RT-PCR 产物之一作为探针，对从盲鳗肝脏 cDNA 文库中分离的全长 cDNA 克隆进行分析，表明该 cDNA 编码盲鳗甘露糖结合凝集素相关丝氨酸蛋白酶 WASP)1。这一证据强烈表明盲鳗至少通过由凝集素途径组成的补体系统来防御病原体

项目成果

水産学シリーズ135 魚類の免疫系

渔业科学系列135 鱼类免疫系统

• 发表时间: 2003
• 作者: Youji Shimazaki;Kazutaka Maeyama;Tamotsu Fujii;Youji Shimazaki;渡辺 翼(編)
• 通讯作者: 渡辺 翼(編)

Host defense system in the cyclostomes.

环口动物的宿主防御系统。

• 发表时间: 2002
• 期刊: Journal of Clinical and Experimental Medicine(IGAKU NO AYUMI) 200
• 作者: Tamotsu Fujii;藤井 保;Tamotsu Fujii
• 通讯作者: Tamotsu Fujii

名取 俊二(編): "動物界における免疫系の進化"医歯薬出版株式会社. 118 (2002)

名取俊二（主编）：《动物界免疫系统的进化》石药出版株式会社 118（2002）

Isolation of the third component and its derivative with anaphylatoxin-like activity from the plasma of the newt Cynops pyrrhogaster.

从蝾螈Cynopspyrrhogaster的血浆中分离出第三种成分及其具有过敏毒素样活性的衍生物。

• 发表时间: 2001
• 期刊: Developmental and Comparative Immunology 25
• 作者: Youji Shimazaki;Kazutaka Maeyama;Tamotsu Fujii
• 通讯作者: Tamotsu Fujii

Isolation of the third component of complement and its derivative with anaphylatoxin-like activity from the plasma of the newt Cynops pyrrhogaster.

从蝾螈Cynopspyrrhogaster血浆中分离出补体的第三种成分及其具有过敏毒素样活性的衍生物。

• 发表时间: 2001
• 期刊: Developmental and Comparative Immunology 25
• 作者: Tamotsu Fujii;藤井 保;Tamotsu Fujii;藤井 保;Youji Shimazaki
• 通讯作者: Youji Shimazaki